Invitrogen

CD154 (CD40 Ligand) Monoclonal Antibody (MR1), PE

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CD154 (CD40 Ligand) Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of CD154 (CD40 Ligand) in PMA and ionomycin stimulated BALB/c mouse splenocytes. Cells were stained withCD154 (CD40 Ligand) Monoclonal Antibody (MR1), PE (Product # HMCD15404), Rat Anti-Mouse CD4-FITC, and Rat Anti-Mouse CD8 alpha-FITC.

Product Details

HMCD15404

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	0.1-0.2 µg / 1x10^6 cells	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Armenian hamster / IgG3
Class	Monoclonal
Type	Antibody
Clone	MR1
Immunogen	Mouse CD154. from Activated mouse Th1 clone D1.6 View immunogen
Conjugate	PE FITC
Excitation/Emission Max	565/576 nm View spectra
Form	Liquid
Concentration	0.1 mg/mL
Purification	purified
Storage buffer	PBS
Contains	<0.1% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2536596

Target Information

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth.

Nature communications

Klein JC,Moses K,Zelinskyy G,Sody S,Buer J,Lang S,Helfrich I,Dittmer U,Kirschning CJ,Brandau S

HMCD15404 was used in flow cytometry to find that TLR3, 7 and 9 deficiencies on host cells result in complete tumor regression and induction of anti-tumor immunity

Thu Mar 16 00:00:00 EDT 2017

Expression and function of 4-1BB and 4-1BB ligand on murine dendritic cells.

International immunology

Futagawa T,Akiba H,Kodama T,Takeda K,Hosoda Y,Yagita H,Okumura K

HMCD15404 was used in flow cytometry to characterize the expression and function of 4-1BBL.

Fri Mar 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: CD154; CD40 ligand; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; sCD40L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5

Gene Aliases: CD154; CD40-L; Cd40l; Cd40lg; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; T-BAM; Tnfsf5; TRAP

UniProt ID: (Mouse) P27548

Entrez Gene ID: (Mouse) 21947

Function(s)

positive regulation of immune response to tumor cell inflammatory response immune response signal transduction platelet activation B cell differentiation positive regulation of B cell proliferation positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production B cell proliferation negative regulation of apoptotic process isotype switching positive regulation of MHC class II biosynthetic process immunoglobulin secretion regulation of immunoglobulin secretion dendritic cell differentiation positive regulation of endothelial cell apoptotic process

Process(es)

cytokine activity tumor necrosis factor receptor binding CD40 receptor binding

CD154 (CD40 Ligand) Monoclonal Antibody (MR1), PE

Product Details

HMCD15404

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

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