|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5 µL (0.25 µg)/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 5 publications below|
Description: The eBio5E8-C7-F10 monoclonal antibody reacts with human CD182 (CXCR2, IL-8R beta). CD182 is a 67-70 kDa member of the seven-transmembrane spanning G-protein coupled receptor (GPCR) family. CD182 is expressed as a homodimer, or a heterodimer with CD181 (CXCR1, IL-8R alpha) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it's ligands, which include IL-8, NAP-2, GCP-2 and GRO alpha, -beta and -gamma, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.
Applications Reported: This eBio5E8-C7-F10 (5E8-C7-F10) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio5E8-C7-F10 (5E8-C7-F10) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
CXCR2 is a receptor for interleukin-8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL-8 with a high affinity and to GRO/MGSA and NAP-2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||The CXC chemokine receptor 2, CXCR2, is the putative receptor for ELR+ CXC chemokine-induced angiogenic activity.||Addison CL,Daniel TO,Burdick MD,Liu H,Ehlert JE,Xue YY,Buechi L,Walz A,Richmond A,Strieter RM||Journal of immunology (Baltimore, Md. : 1950) (165:5269)||2000|
|Not Applicable||Not Cited||The biologic role of interleukin-8: functional analysis and expression of CXCR1 and CXCR2 on human eosinophils.||Petering H,Götze O,Kimmig D,Smolarski R,Kapp A,Elsner J||Blood (93:694)||1999|
|Not Applicable||Not Cited||Chemokine antagonists that discriminate between interleukin-8 receptors. Selective blockers of CXCR2.||Jones SA,Dewald B,Clark-Lewis I,Baggiolini M||The Journal of biological chemistry (272:16166)||1997|
|Not Applicable||Not Cited||Monoclonal antibodies detect different distribution patterns of IL-8 receptor A and IL-8 receptor B on human peripheral blood leukocytes.||Chuntharapai A,Lee J,Hébert CA,Kim KJ||Journal of immunology (Baltimore, Md. : 1950) (153:5682)||1994|
|Not Applicable||Not Cited||Structure, genomic organization, and expression of the human interleukin-8 receptor B gene.||Sprenger H,Lloyd AR,Lautens LL,Bonner TI,Kelvin DJ||The Journal of biological chemistry (269:11065)||1994|