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          • Primary Antibodies ›
          • BCL-2 Antibodies

          Proteintech

          Bcl2 Monoclonal Antibody (1B5B9), CoraLite® Plus 647

          View all (170) BCL-2 antibodies

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          Cite Bcl2 Monoclonal Antibody (1B5B9), CoraLite® Plus 647

          Additional Information:
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          • Antibody Testing Data (1)
          Bcl2 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          Bcl2 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Bcl2 Antibody (CL647-66799) in Flow

          1X10^6 NIH/3T3 cells were intracellularly stained with 0.2 µg CoraLite® Plus 647 Anti-Mouse Bcl2 (CL647-66799, Clone:1B5B9) (red), or 0.2 µg APC-65128 ; APC Mouse IgG2b Isotype Control (APC-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Bcl2 Antibody in Flow Cytometry (Flow)
          Bcl2 Monoclonal Antibody (1B5B9), CoraLite® Plus 647

          Product Details

          CL647-66799

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cell suspension
          -
          Product Specifications

          Species Reactivity

          Mouse

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1B5B9

          Immunogen

          Recombinant protein
          3D Epitope / Immunogen

          Conjugate

          CoraLite® Plus 647 CoraLite® Plus 647 CoraLite® Plus 647
          • Unconjugated
          • CoraLite 555
          • CoraLite Plus 488
          • Request custom conjugation

          Excitation/Emission Max

          654/674 nm

          Form

          Liquid

          Purification

          Protein G

          Storage buffer

          PBS with 50% glycerol, 0.5% BSA

          Contains

          0.05% ProClin 300

          Storage conditions

          -20°C, store in dark

          Shipping conditions

          Wet ice

          Product Specific Information

          Immunogen sequence: MAQAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDADAAPL GAAPTPGIFS FQPESNPMPA VHRDMAARTS PLRPLVATAG PALSPVPPVV HLTLRRAGDD FSRRYRRDFA EMSSQLHLTP FTARGRFATV VEELFRDGVN WGRIVAFFEF GGVMCVESVN REMSPLVDNI ALWMTEYLNR HLHTWIQDNG GWDAFVELYG PSMRPLFDFS WLSLKTLLSL ALVGACITLG AYLGHK

          Target Information

          BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Apoptosis regulator Bcl-2; B-cell leukemia/lymphoma 2; Bcl 2; OTTHUMP00000163680

          View more View less

          Gene Aliases: AW986256; Bcl-2; Bcl2; C430015F12Rik; D630044D05Rik; D830018M01Rik

          View more View less

          UniProt ID: (Mouse) P10417

          View more View less

          Entrez Gene ID: (Mouse) 12043

          View more View less

          Function(s)
          protease binding protein binding transcription factor binding channel activity channel inhibitor activity protein phosphatase binding ubiquitin protein ligase binding identical protein binding protein homodimerization activity protein heterodimerization activity BH domain binding BH3 domain binding protein phosphatase 2A binding
          Process(es)
          protein polyubiquitination cell morphogenesis response to acid chemical ossification ovarian follicle development metanephros development ureteric bud development branching involved in ureteric bud morphogenesis behavioral fear response leukocyte homeostasis B cell homeostasis kidney development release of cytochrome c from mitochondria regulation of cell-matrix adhesion lymphocyte homeostasis lymphoid progenitor cell differentiation B cell lineage commitment T cell lineage commitment immune system development response to ischemia renal system process protein dephosphorylation melanin metabolic process regulation of nitrogen utilization cellular calcium ion homeostasis apoptotic process cellular response to DNA damage stimulus response to oxidative stress actin filament organization axonogenesis cell aging cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation male gonad development extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage intrinsic apoptotic signaling pathway in response to oxidative stress apoptotic mitochondrial changes response to toxic substance post-embryonic development organ morphogenesis response to iron ion response to UV-B response to gamma radiation regulation of gene expression negative regulation of autophagy negative regulation of calcium ion transport into cytosol regulation of glycoprotein biosynthetic process mesenchymal cell development positive regulation of neuron maturation positive regulation of smooth muscle cell migration cell growth single organismal cell-cell adhesion peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cochlear nucleus development gland morphogenesis regulation of transmembrane transporter activity hemopoiesis B cell differentiation T cell differentiation negative regulation of ossification positive regulation of cell growth negative regulation of cell growth melanocyte differentiation negative regulation of cell migration positive regulation of B cell proliferation hair follicle morphogenesis axon regeneration regulation of protein stability endoplasmic reticulum calcium ion homeostasis glomerulus development negative regulation of cellular pH reduction regulation of protein localization negative regulation of myeloid cell apoptotic process T cell differentiation in thymus positive regulation of peptidyl-serine phosphorylation negative regulation of osteoblast proliferation response to cytokine response to nicotine organ growth growth positive regulation of multicellular organism growth B cell proliferation cellular response to glucose starvation response to drug response to hydrogen peroxide regulation of apoptotic process T cell homeostasis negative regulation of apoptotic process regulation of programmed cell death positive regulation of catalytic activity CD8-positive, alpha-beta T cell lineage commitment pigmentation regulation of protein homodimerization activity regulation of protein heterodimerization activity negative regulation of neuron apoptotic process ear development regulation of viral genome replication positive regulation of melanocyte differentiation negative regulation of mitotic cell cycle negative regulation of retinal cell programmed cell death regulation of mitochondrial membrane permeability focal adhesion assembly developmental pigmentation regulation of developmental pigmentation positive regulation of developmental pigmentation spleen development thymus development response to steroid hormone digestive tract morphogenesis developmental growth oocyte development positive regulation of skeletal muscle fiber development pigment granule organization homeostasis of number of cells within a tissue regulation of catalytic activity B cell receptor signaling pathway response to glucocorticoid neuron apoptotic process defense response to virus regulation of cell cycle regulation of mitochondrial membrane potential regulation of calcium ion transport transmembrane transport intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to organic substance cellular response to hypoxia reactive oxygen species metabolic process extrinsic apoptotic signaling pathway in absence of ligand negative regulation of G1/S transition of mitotic cell cycle negative regulation of reactive oxygen species metabolic process negative regulation of anoikis negative regulation of apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.

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