Invitrogen

CaV1.2 Monoclonal Antibody (S57), PerCP

View all (24) CaV1.2 antibodies
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

MA5-45389

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:1000
-

Immunocytochemistry (ICC/IF)

1:100
-

Immunoprecipitation (IP)

1:200
-

Antibody Microarray (AM)

Assay-dependent
-
Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

S57

Immunogen

Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2
View immunogen

Conjugate

PerCP PerCP PerCP

Excitation/Emission Max

482/675 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

47.35mM phosphate/2.15mM MES, pH 7.4, with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931843

Product Specific Information

1 µg/mL of MA5-45389 was sufficient for detection of Cav1.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 240kDa (varies with cell background due to glycosylation).

Target Information

CACNA1C encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by CACNA1C binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: brain class C; CACH3; CACN4; CACNA 1D; CACNL1A2; calcium channel voltage-dependent alpha1c subunit; calcium channel, cardic dihydropyridine-sensitive, alpha-1 subunit; Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle; calcium channel, voltage-dependent, alpha 1C subunit; calcium channel, voltage-dependent, L type, alpha 1C subunit; DHP receptor; DHPR, alpha-1 subunit; L-type calcium channel alpha-1 subunit; long-NT; LTCC; MBC; MELC-CC; MGC120730; Mouse brain class C; neuronal voltage-gated calcium channel alpha 1C subunit; Rat brain class C; RBC; skeletal muscle-specific calcium channel; voltage-dependent L-type Ca2+ channel alpha 1 subunit; Voltage-dependent L-type calcium channel subunit alpha-1C; Voltage-gated calcium channel subunit alpha Cav1.2; voltage-gated L-type calcium channel Cav1.2 alpha 1 subunit, splice variant 10*

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Gene Aliases: CACH2; CACN2; CACNA1C; CACNL1A1; CaV1.2; CCHL1A1; D930026N18Rik; I79_006562; LQT8; MBC; MELC-CC; RATIVS302; TS

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UniProt ID: (Human) Q13936, (Mouse) Q01815, (Rat) P22002

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Entrez Gene ID: (Human) 775, (Mouse) 12288, (Chinese hamster) 100754788, (Rat) 24239

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Function(s)
voltage-gated calcium channel activity protein binding calmodulin binding high voltage-gated calcium channel activity metal ion binding alpha-actinin binding voltage-gated calcium channel activity involved in cardiac muscle cell action potential voltage-gated calcium channel activity involved in AV node cell action potential ion channel activity voltage-gated ion channel activity calcium channel activity enzyme binding protein domain specific binding ion channel binding protein phosphatase 2A binding voltage-gated ion channel
Process(es)
immune system development positive regulation of cytosolic calcium ion concentration heart development regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion embryonic forelimb morphogenesis calcium-mediated signaling using extracellular calcium source camera-type eye development regulation of insulin secretion calcium ion transport into cytosol cardiac conduction calcium ion transmembrane transport via high voltage-gated calcium channel calcium ion transmembrane transport cardiac muscle cell action potential involved in contraction membrane depolarization during cardiac muscle cell action potential membrane depolarization during AV node cell action potential cell communication by electrical coupling involved in cardiac conduction regulation of heart rate by cardiac conduction regulation of ventricular cardiac muscle cell action potential membrane depolarization during atrial cardiac muscle cell action potential transport ion transport calcium ion transport cellular calcium ion homeostasis smooth muscle contraction chemical synaptic transmission adult walking behavior regulation of blood pressure visual learning calcium ion regulated exocytosis regulation of vasoconstriction insulin secretion growth hormone secretion regulation of ion transmembrane transport glucose homeostasis regulation of organ growth transmembrane transport smooth muscle contraction involved in micturition calcium ion import membrane depolarization during action potential
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