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Agrisera
D14/Strigolactone esterase D14 Polyclonal Antibody
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FIGURE: 1 / 1
D14/Strigolactone esterase D14 Antibody (AS16-3694) in WB
60 µg of soluble protein from seven-day-old Arabidopsis thaliana seedlings (grown under long day conditions on MS agar plates) extracted with PE buffer (50 m M TRIS p H 7.5,150 m M Na Cl, 10% glycerol, 0.1% Tween-20,1 m M DTT, 1 m M PMSF, 1 x Complete protease inhibitor (Roche)) and denatured with LaemLli buffer (including 125 m M DTT) at 95°C for 5 min. The samples were separated on 12% SDS-PAGE and blotte... View More
Product Details
AS16-3694
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana D14.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
D14, also called DWARF14, is a plant alpha beta hydrolase that functions as the receptor for the hormone strigolactone, a carotenoid derived signal regulating shoot branching, root architecture, and responses to environmental cues. Upon binding strigolactone, D14 undergoes a conformational change and hydrolyzes the ligand, enabling formation of a signaling complex with the F box protein D3 or MAX2 and repressors such as D53 or SMXLs, which are then targeted for ubiquitin mediated degradation. This triggers transcriptional and developmental programs that suppress axillary bud outgrowth, modulate auxin transport, and adjust resource allocation. D14 activity integrates nutrient status, mycorrhizal interactions, and stress signals, and its mutants show increased tillering or branching and altered root traits. D14 works with biosynthetic genes like D27, CCD7, and CCD8 and with perception components in the nucleus to fine tune growth and adaptation across plant species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dwarf 88 esterase; DWARF14; HTD2; probable strigolactone esterase D14; Protein DWARF-14; Protein DWARF-88; Protein HIGH-TILLERING DWARF 2; strigolactone esterase D14
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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