Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of EGF (green) in HeLa cells either left untreated (left panel) or treated with 10ng/ml EGF (right panel) for 5 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an EGF monoclonal antibody (Product # M806) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Recombinant Human EGF|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.125 - 0.25 µg/ml|
|Western Blot (WB)||1:1000 - 1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The M806 anti-EGF antibody (Clone 3A8) has successfully been paired as the detection antibody in a sandwich ELISA with coating antibody M805 (Clone 1H11).
Typical dilutions for sandwich ELISA include 1-3 µg/ml for coating and 0.125-0.5 µg/ml for detection.
The protein encoded by EGF gene is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
beta-urogastrone, pro-epidermal growth factor, HMGF (human milk growth factor), PGF (prostatic growth factor), tooth-lid factor, Urogastrone