|ELISA (ELISA)||0.125 - 0.25 µg/ml|
|Western Blot (WB)||1:1000 - 1:5000|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Recombinant Human EGF|
The M806 anti-EGF antibody (Clone 3A8) has successfully been paired as the detection antibody in a sandwich ELISA with coating antibody M805 (Clone 1H11).
Typical dilutions for sandwich ELISA include 1-3 µg/ml for coating and 0.125-0.5 µg/ml for detection.
The protein encoded by EGF gene is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: beta-urogastrone; EGF; HMGF (human milk growth factor); PGF (prostatic growth factor); Pro-epidermal growth factor; tooth-lid factor; Urogastrone
Gene Aliases: EGF; HOMG4; URG
UniProt ID: (Human) P01133
Entrez Gene ID: (Human) 1950
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.