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Invitrogen
CD91 Monoclonal Antibody (A2MR-a2), NovaFluor™ Red 725, eBioscience™
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Product Details
H073T03R05-A
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
A2MR-a2
Conjugate
Excitation/Emission Max
636/727 nm
View spectra
Form
Liquid
Concentration
0.05 µg/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
RRID
Product Specific Information
Description
The A2MR-a2 monoclonal antibody reacts with human CD91, also known as LRP-1 and alpha-2 macroglobulin receptor.
This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.
Applications Tested
This A2MR-a2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.05 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Master mixes
• Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time.
• We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer.
Whole Blood compatibility
• When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates.
• See the Bulk Lysis of Human Whole Blood protocol here.
• Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-than-expected background staining.
Viability dye compatibility
• NovaFluor dyes are not compatible with DNA intercalating viability dyes.
• Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
CellBlox Plus Blocking Buffer
• This NovaFluor conjugate comes with CellBlox Plus Blocking Buffer (for 25 µg or 25 test products, Cat. No. C001T02F01; for 100 µg or 100 test, Cat. No. C001T03F01), essential for optimal staining.
• Use CellBlox Plus Blocking Buffer in all experiments with NovaFluor conjugates.
• Add 5 µL per sample to antibody cocktails/master mixes (regardless of how many Novafluor-conjugated antibodies are present) before combining with cells.
• CellBlox Plus Blocking Buffer is compatible with either Super Bright Complete Blocking Buffer or Brilliant Stain Buffer and can be used in antibody cocktails/master mixes with those reagents.
• For single-color controls, use 5 µL of CellBlox Plus Blocking Buffer per 100 µL of cell sample (10^3 to 10^8 cells).
NovaFluor conjugates are based on Phiton technology utilizing novel fluorophore-containing nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 636 nm; Emission: 727 nm; Laser: 633-640 nm (Red) Laser
Target Information
CD91 is a 600 kDa type I transmembrane protein composed of an alpha and beta chain. The alpha chain interacts with a variety of structurally and functionally diverse ligands, while the beta chain contains two tyrosine phosphorylation motifs that interact with adaptor and signaling proteins. This receptor is expressed by cells in the monocytic lineage, dendritic cells, hepatocytes, fibroblasts, adipocytes, neurons, and syncytiotrophoblasts. CD91 plays a crucial role in several cellular processes, including ligand endocytosis and trafficking to lysosomes, intracellular signaling, lipid homeostasis, and clearance of apoptotic cells. It is involved in cross-presenting antigens via MHC class I molecules on antigen-presenting cells after endocytosis of heat shock protein-peptide complexes. Additionally, CD91 is essential for the A2M-mediated clearance of secreted amyloid precursor protein and beta-amyloid, the main component of amyloid plaques found in Alzheimer patients. Expression of CD91 decreases with age and is found to be lower in brain tissue from Alzheimer patients compared to controls. The receptor also plays a role in cell migration and activating signaling cascades, highlighting its importance in both immune function and disease processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
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Bioinformatics
Protein Aliases: A2MR; Alpha-2-macroglobulin receptor; APOER; Apolipoprotein E receptor; CD91; FLJ16451; low density lipoprotein receptor-related protein 1; LRP-1; MGC88725; Prolow-density lipoprotein receptor-related protein 1; TbetaR-V/LRP-1/IGFBP-3 receptor; type V tgf-beta receptor
Gene Aliases: A2MR; APOER; APR; CD91; DDH3; IGFBP-3R; IGFBP3R; IGFBP3R1; KPA; LRP; LRP1; LRP1A; TGFBR5
UniProt ID: (Human) Q07954
Entrez Gene ID: (Human) 4035
beta-amyloid binding
RNA binding
low-density lipoprotein receptor activity
scavenger receptor activity
calcium ion binding
protein binding
alpha-2 macroglobulin receptor activity
apolipoprotein receptor activity
clathrin heavy chain binding
apolipoprotein binding
signaling receptor activity
cargo receptor activity
heparan sulfate proteoglycan binding
macromolecular complex binding
metal ion binding
lipoprotein particle receptor binding
retinoid metabolic process
astrocyte activation involved in immune response
lipid metabolic process
endocytosis
receptor-mediated endocytosis
phagocytosis
lysosomal transport
enzyme linked receptor protein signaling pathway
negative regulation of macromolecule metabolic process
negative regulation of gene expression
regulation of extracellular matrix disassembly
positive regulation of cholesterol efflux
negative regulation of smooth muscle cell migration
regulation of endocytosis
negative regulation of Wnt signaling pathway
receptor internalization
positive regulation of lipid transport
regulation of actin cytoskeleton organization
aorta morphogenesis
lipoprotein transport
apoptotic cell clearance
transcytosis
positive regulation of endocytosis
positive regulation of transport
regulation of protein metabolic process
negative regulation of SMAD protein import into nucleus
beta-amyloid clearance
amyloid-beta clearance by transcytosis
amyloid-beta clearance by cellular catabolic process
transport across blood-brain barrier
positive regulation of beta-amyloid clearance
regulation of extracellular matrix organization
positive regulation of reverse cholesterol transport
positive regulation of protein localization to plasma membrane
positive regulation of transcytosis
cellular response to beta-amyloid
positive regulation of lysosomal protein catabolic process
negative regulation of platelet-derived growth factor receptor-beta signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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