This Antibody was verified by Cell Treatment to ensure that the antibody binds to the antigen stated. View Details
Description: This Mgc3 monoclonal antibody detects hypoxia-inducible factor 1 alpha (HIF-1 alpha) from human, non-human primate, bovine, mouse and porcine cells. This antibody does not cross-react with ARNT or the related HIF-2 alpha.
Mgc3 has been successfully used in western blotting, immunofluorescence, immunocytochemistry, immunoprecipitation, flow cytometry and gel shift procedures. By western blot, this antibody detects a ~116 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells. Immunofluorescence staining of HIF-1 alpha in COS-7 cells with clone Mgc3 yields nuclear staining after exposing cells to 1% oxygen for 4 hours. In gel shift assay experiments, the Mgc3 antibody detected only mouse and not human HIF-1 alpha.
Applications Reported: This Mgc3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This Mgc3 antibody has been tested by flow cytometric analysis of DFOA stimulated HeLa cells using the methanol fixation protocol. This may be used at less than or equal to 1.0 µg/mL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
HIF1 alpha functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, cell proliferation, tumor angiogenesis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 alpha is regulated post-translationally by Factors Inhibiting HIF (FIH) and Prolyl Hydroxylase (PHD's). Once accumulated HIF1 alpha translocates into the nucleus and binds to its heterodynamic binding partners, ARNT and p300, facilitating expression of genes involved in the hypoxic response. Dysregulation of HIF1 alpha signaling has been observed in several tumor types. Hypoxia contributes significantly to the pathophysiology of multiple human diseases, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and chronic obstructive pulmonary disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ARNT interacting protein; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8
Gene Aliases: AA959795; BHLHE78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; HIF1A; HIF1alpha; MOP1; PASD8