Invitrogen

HLA-E Monoclonal Antibody (3D12HLA-E), PE, eBioscience™

Product Image Gallery

HLA-E Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Anti-Human HLA-ABC FITC (Product # 11-9983-42) and Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human HLA-E PE (right). Cells in the lymphocyte gate were used for analysis.

Product Details

12-9953-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.5 µg)/test	View 14 publications 14 publications

Product Specifications
Species Reactivity	Human
Published species	Human, Rat
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	3D12HLA-E
Conjugate	PE Alexa Fluor 488 APC Functional Grade Unconjugated
Excitation/Emission Max	565/576 nm View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_1834461

Product Specific Information

Description: The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.

Applications Reported: This 3D12 (HLA-E) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 3D12HLA-E antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Vγ9Vδ2 T Cells Activation Through Phosphoantigens Can Be Impaired by a RHOB Rerouting in Lung Cancer.

Frontiers in immunology

Laplagne C,Meddour S,Figarol S,Michelas M,Calvayrac O,Favre G,Laurent C,Fournié JJ,Cabantous S,Poupot M

12-9953-42 was used in Flow Cytometry to study T cell activation in lung cancer.

Tue Apr 06 00:00:00 EDT 2021

Selection of tumor‑resistant variants following sustained natural killer cell‑mediated immune stress.

Oncology reports

Carré T,Thiery J,Janji B,Terry S,Gros G,Meurice G,Kieda C,Olive D,Chouaib S

12-9953 was used in Flow cytometry/Cell sorting to study the role of NK cells which may span far beyond the mere killing of malignant cells, and NK cells may be important effectors during cancer immunoediting.

Mon Feb 01 00:00:00 EST 2021

Therapeutic CD94/NKG2A blockade improves natural killer cell dysfunction in chronic lymphocytic leukemia.

Oncoimmunology

McWilliams EM,Mele JM,Cheney C,Timmerman EA,Fiazuddin F,Strattan EJ,Mo X,Byrd JC,Muthusamy N,Awan FT

Tue Jan 12 00:00:00 EST 2021

Non-classical HLA-class I expression in serous ovarian carcinoma: Correlation with the HLA-genotype, tumor infiltrating immune cells and prognosis.

Oncoimmunology

Andersson E,Poschke I,Villabona L,Carlson JW,Lundqvist A,Kiessling R,Seliger B,Masucci GV

12-9953 was used in Flow cytometry/Cell sorting to investigate the expression of HLA -G and -E on serous ovarian carcinoma tumour cells along with the infiltration of immune cells in the tumour microenvironment.

Tue Jan 12 00:00:00 EST 2021

Effects of anti-NKG2A antibody administration on leukemia and normal hematopoietic cells.

Haematologica

Ruggeri L,Urbani E,André P,Mancusi A,Tosti A,Topini F,Bléry M,Animobono L,Romagné F,Wagtmann N,Velardi A

12-9953 was used in Flow cytometry/Cell sorting to investigate the potential clinical role of a novel, humanised anti-NKG2A monoclonal antibody in vitro and in humanised mouse models.

Sun May 01 00:00:00 EDT 2016

Adaptive Immune Responses in a Multiple Sclerosis Patient with Acute Varicella-Zoster Virus Reactivation during Treatment with Fingolimod.

International journal of molecular sciences

Harrer A,Wipfler P,Pilz G,Oppermann K,Haschke-Becher E,Afazel S,Kraus J,Trinka E,Sellner J

12-9953 was used in Flow cytometry/Cell sorting to investigate any potential risks to patients receiving fingolimod during varicella zoster virus infection.

Thu Sep 10 00:00:00 EDT 2015

Highly effective NK cells are associated with good prognosis in patients with metastatic prostate cancer.

Oncotarget

Pasero C,Gravis G,Granjeaud S,Guerin M,Thomassin-Piana J,Rocchi P,Salem N,Walz J,Moretta A,Olive D

12-9953 was used in Flow cytometry/Cell sorting to identify natural killer cells as potential predictive biomarkers to stratify patients who are likely to have longer castration response.

Wed Jun 10 00:00:00 EDT 2015

Dynamic contrast enhanced MRI detects early response to adoptive NK cellular immunotherapy targeting the NG2 proteoglycan in a rat model of glioblastoma.

PloS one

Rygh CB,Wang J,Thuen M,Gras Navarro A,Huuse EM,Thorsen F,Poli A,Zimmer J,Haraldseth O,Lie SA,Enger PØ,Chekenya M

12-9953 was used in Flow cytometry/Cell sorting to examine whether multiparametric, longitudinal magnetic resonance imaging of physiological parameters and pharmacokinetic models might detect early biological responses to immunotherapy for glioblastoma targeting NG2/CSPG4 with mAb9.2.27 and natural killer cells.

Wed Jun 10 00:00:00 EDT 2015

Human NK cells activated by EBV⁺ lymphoblastoid cells overcome anti-apoptotic mechanisms of drug resistance in haematological cancer cells.

Oncoimmunology

Sánchez-Martínez D,Azaceta G,Muntasell A,Aguiló N,Núñez D,Gálvez EM,Naval J,Anel A,Palomera L,Vilches C,Marzo I,Villalba M,Pardo J

12-9953 was used in Flow cytometry/Cell sorting to demonstrate a novel mechanism for natural killer cells to overcome oncogenic alterations that allow haematological cancer cells to evade destruction.

Sun Mar 01 00:00:00 EST 2015

The combination of type I IFN, TNF-α, and cell surface receptor engagement with dendritic cells enables NK cells to overcome immune evasion by dengue virus.

Journal of immunology (Baltimore, Md. : 1950)

Lim DS,Yawata N,Selva KJ,Li N,Tsai CY,Yeong LH,Liong KH,Ooi EE,Chong MK,Ng ML,Leo YS,Yawata M,Wong SB

12-9953 was used in Flow cytometry/Cell sorting to investigate the response of NK cells against dengue-infected cells, showing that a combination of type I IFN, TNF-α, and cell surface receptor engagement with dendritic cells is required.

Sat Nov 15 00:00:00 EST 2014

NK cell activation in human hantavirus infection explained by virus-induced IL-15/IL15Rα expression.

PLoS pathogens

Braun M,Björkström NK,Gupta S,Sundström K,Ahlm C,Klingström J,Ljunggren HG

12-9953 was used in Flow cytometry/Cell sorting to investigate the mechanisms behind the increased natural killer cell activation and proliferation in Puumala hantavirus infection in humans.

Sat Nov 01 00:00:00 EDT 2014

Synergistic inhibition of natural killer cells by the nonsignaling molecule CD94.

Proceedings of the National Academy of Sciences of the United States of America

Cheent KS,Jamil KM,Cassidy S,Liu M,Mbiribindi B,Mulder A,Claas FH,Purbhoo MA,Khakoo SI

12-9953 was used in Flow cytometry/Cell sorting to elucidate the benefits of having two distinct inhibitory receptor-ligand systems to inhibit natural killer cells.

Tue Oct 15 00:00:00 EDT 2013

Transcription of preintegrated HIV-1 cDNA modulates cell surface expression of major histocompatibility complex class I via Nef.

Journal of virology

Sloan RD,Kuhl BD,Donahue DA,Roland A,Bar-Magen T,Wainberg MA

12-9953 was used in Flow cytometry/Cell sorting to suggest that Nef-mediated evasion of host immune responsiveness might be attributable to transcription from unintegrated viral DNA.

Tue Mar 01 00:00:00 EST 2011

The rapid induction of HLA-E is essential for the survival of antigen-activated naive CD4 T cells from attack by NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Takao S,Ishikawa T,Yamashita K,Uchiyama T

12-9953 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which NK cells regulate adaptive immunity, showing that rapid induction of HLA-E is essential for the survival of antigen-activated naive CD4 T cells.

Mon Nov 15 00:00:00 EST 2010

Bioinformatics

Protein Aliases: DAMA-277I14.1; DAQB-90C11.16; DKFZp686P19218; FLJ26655; HLA class I histocompatibility antigen, alpha chain E; HLA class I histocompatibility antigen, E alpha chain; lymphocyte antigen; MHC 1; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC1; sHLA-E; Soluble HLA class I histocompatibility antigen, alpha chain E

Gene Aliases: EA1.2; EA2.1; HLA-6.2; HLA-E; HLAE; MHC; QA1

UniProt ID: (Human) P13747

Entrez Gene ID: (Human) 3133

Molecular Function: major histocompatibility complex protein

Function(s)

positive regulation of T cell mediated cytotoxicity adaptive immune response antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class Ib antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent regulation of natural killer cell mediated immunity positive regulation of natural killer cell mediated immunity antibacterial humoral response positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of TRAIL production positive regulation of tumor necrosis factor production CD8-positive, alpha-beta T cell activation protection from natural killer cell mediated cytotoxicity innate immune response regulation of immune response defense response to Gram-positive bacterium positive regulation of immunoglobulin secretion interferon-gamma-mediated signaling pathway type I interferon signaling pathway positive regulation of CD8-positive, alpha-beta T cell proliferation

Process(es)

receptor binding beta-2-microglobulin binding MHC class I protein binding peptide antigen binding natural killer cell lectin-like receptor binding

HLA-E Monoclonal Antibody (3D12HLA-E), PE, eBioscience™

Product Details

12-9953-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

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