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Invitrogen
IL-6 Monoclonal Antibody (MQ2-13A5), eFluor™ 450, eBioscience™
FIGURE: 1 / 12
IL-6 Antibody (48-7069-42) in Flow
Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with LPS (right) in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03). Cells were then fixed and permeabilized with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and intracellularly stained with Anti-Human CD14 APC (Product # 17-0149-42) and Anti-Human IL-6 eFluor® 450. Cells in the monocyte gate were used for analysis.
Product Details
48-7069-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MQ2-13A5
Conjugate
Excitation/Emission Max
405/445 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574105
Product Specific Information
Description: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.
Applications Reported: This MQ2-13A5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This MQ2-13A5 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6
Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6
UniProt ID: (Human) P05231
Entrez Gene ID: (Human) 3569
neutrophil apoptotic process
response to yeast
hepatic immune response
neutrophil mediated immunity
monocyte chemotaxis
positive regulation of acute inflammatory response
positive regulation of leukocyte chemotaxis
negative regulation of protein kinase activity
acute-phase response
inflammatory response
humoral immune response
aging
positive regulation of cell proliferation
negative regulation of cell proliferation
regulation of cell shape
response to heat
response to cold
regulation of vascular endothelial growth factor production
positive regulation of gene expression
negative regulation of lipid storage
response to auditory stimulus
cell growth
cytokine-mediated signaling pathway
platelet activation
response to caffeine
endocrine pancreas development
neuron projection development
response to nutrient levels
response to peptidoglycan
positive regulation of chemokine production
positive regulation of interleukin-6 production
response to insulin
negative regulation of collagen biosynthetic process
positive regulation of peptidyl-serine phosphorylation
positive regulation of protein import into nucleus, translocation
cellular response to hepatocyte growth factor stimulus
positive regulation of T cell proliferation
response to drug
positive regulation of tyrosine phosphorylation of Stat3 protein
glucose homeostasis
defense response to protozoan
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
response to amino acid
positive regulation of MAPK cascade
negative regulation of chemokine biosynthetic process
regulation of circadian sleep/wake cycle, non-REM sleep
positive regulation of nitric oxide biosynthetic process
cell redox homeostasis
negative regulation of fat cell differentiation
positive regulation of T-helper 2 cell differentiation
positive regulation of neuron differentiation
positive regulation of osteoblast differentiation
negative regulation of gluconeogenesis
positive regulation of translation
positive regulation of DNA replication
regulation of angiogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
response to antibiotic
muscle cell cellular homeostasis
bone remodeling
negative regulation of hormone secretion
negative regulation of muscle organ development
positive regulation of smooth muscle cell proliferation
positive regulation of epithelial cell proliferation
negative regulation of cytokine secretion
positive regulation of peptidyl-tyrosine phosphorylation
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
positive regulation of B cell activation
positive regulation of immunoglobulin secretion
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of NF-kappaB transcription factor activity
response to glucocorticoid
response to calcium ion
response to electrical stimulus
defense response to virus
positive regulation of protein kinase B signaling
positive regulation of transmission of nerve impulse
branching involved in salivary gland morphogenesis
epithelial cell proliferation involved in salivary gland morphogenesis
glucagon secretion
interleukin-6-mediated signaling pathway
cellular response to hydrogen peroxide
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to dexamethasone stimulus
positive regulation of cell proliferation in bone marrow
T-helper 17 cell lineage commitment
negative regulation of neuron death
positive regulation of STAT protein import into nucleus
positive regulation of type B pancreatic cell apoptotic process
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