Invitrogen

IL-6 Monoclonal Antibody (MQ2-13A5), eFluor™ 450, eBioscience™

Product Image Gallery

IL-6 Antibody in Flow Cytometry (Flow) — Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with LPS (right) in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03). Cells were then fixed and permeabilized with the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and intracellularly stained with Anti-Human CD14 APC (Product # 17-0149-42) and Anti-Human IL-6 eFluor® 450. Cells in the monocyte gate were used for analysis.

IL-6 Antibody in Immunocytochemistry (ICC/IF) — Published figure using IL-6 monoclonal antibody (Product # 48-7069-42) in Immunocytochemistry

IL-6 Antibody in ELISA (ELISA) — Figure 4 Survival and phagocytosis are reduced in PLCgamma2 Ko iPSC macrophages, while LPS-induced secretion of inflammatory cytokines TNFalpha and IL-6 is unaffected. ( A ) Cell death is slightly increased under normal culture conditions after >= 7 days in the PLCgamma2 Ko line compared to the Parent. n = 3 ( B ) In the absence of M-CSF, PLCgamma2 Ko cells show enhanced sensitivity to cell death, as the percentage of dead cells is increasing earlier than in the Parent line. n = 3 ( C ) Representative live-cell images of human iPSC macrophages phagocytosing pHrodo-labelled synaptosomes and quantification of phagocytic uptake indicated as number of spots per cell. PLCgamma2 Ko cells show decreased phagocytosis rate compared to Parent. Cytochalasin D (cytoD, 10 uM) pre-incubation prevents phagocytic uptake in both lines. n = 4. Scale bar 50 mum, inset is a section of the image magnified threefold. ( D) Representative live-cell images of macrophages phagocytosing pHrodo-labelled dead SHSY5Y and quantification of phagocytic uptake indicated as number of spots per cell. PLCgamma2 Ko cells show decreased phagocytosis rate compared to Parent. Cytochalasin D pre-incubation prevents phagocytic uptake in both lines. n = 4. Scale bar 50 mum, inset is a section of the image magnified threefold. ( E) Secretion of TNFalpha and IL-6 after 24 h stimulation with LPS is not affected in the absence of PLCgamma2, n = 4. n.d. - not detectable. Data shown represent mean +- SEM, two-way ANOVA follo

IL-6 Antibody in T-Cell Activation (TCA) — Fig. 2 IL-6 is a downstream mediator of DAC-induced morphological alterations in an NOTCH1 dependent and independent manner a ICN1 transient overexpression increased IL-6 release in cell supernatants at 72 h. The Y axis indicates IL-6 in pg/ml normalized for 1 x 10 6 cells from triplicate experiments and technical duplicates with error bars indicating standard error of means. b Bladder tumor cells were treated with DAC every 48 h for up to 5 days. DAC caused IL-6 release in bladder tumor cell lines with a robust increase in HT1376 cells. The Y-axis indicates IL-6 in pg/ml normalized for 1 x 10 6 cells from triplicate experiments and technical duplicates with error bars indicating standard error of means. c-e mRNA levels of double-stranded RNA sensors increased in DAC-treated cells. The Y-axis indicates average fold change over control cells from triplicate experiments and technical duplicates with error bars indicating standard error of means. Student's t-test was used to compare control and treated cells. * p < 0.05, ** p < 0.01. f Bright field images of Giemsa staining showed morphological changes in cells with exogenous IL-6. Cells with exogenous IL-6 appeared enlarged and flattened as compared to control cells. The addition of IL-6 monoclonal antibody reduced the appearance of DAC-induced enlarged cell morphology. _________ represents 10 um. g The addition of a monoclonal antibody against IL-6 with DAC treatment inhibited IL-6 release as compared to DAC treatment alone. T

Product Details

48-7069-42

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	5 µL (0.125 µg)/test	View 3 publications 3 publications
ELISA (ELISA)	-	View 2 publications 2 publications
T-Cell Activation (TCA)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Rat / IgG1, kappa
Recommended Isotype Control	Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 450, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	MQ2-13A5
Conjugate	eFluor™ 450 Alexa Fluor 700 APC FITC Functional Grade PE PE-Cyanine7 PerCP-eFluor 710 Unconjugated
Excitation/Emission Max	405/445 nm View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2574105

Product Specific Information

Description: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported: This MQ2-13A5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This MQ2-13A5 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

PLCγ2 regulates TREM2 signalling and integrin-mediated adhesion and migration of human iPSC-derived macrophages.

Scientific reports

Obst J,Hall-Roberts HL,Smith TB,Kreuzer M,Magno L,Di Daniel E,Davis JB,Mead E

Published figure using IL-6 monoclonal antibody (Product # 48-7069-42) in ELISA

Wed Oct 06 00:00:00 EDT 2021

Interleukin 6 regulates the expression of programmed cell death ligand 1 in thyroid cancer.

Cancer science

Zhang GQ,Jiao Q,Shen CT,Song HJ,Zhang HZ,Qiu ZL,Luo QY

Published figure using IL-6 monoclonal antibody (Product # 48-7069-42) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Decitabine, a DNA-demethylating agent, promotes differentiation via NOTCH1 signaling and alters immune-related pathways in muscle-invasive bladder cancer.

Cell death & disease

Ramakrishnan S,Hu Q,Krishnan N,Wang D,Smit E,Granger V,Rak M,Attwood K,Johnson C,Morrison C,Pili R,Chatta G,Guru K,Gueron G,McNally L,Wang J,Woloszynska-Read A

Published figure using IL-6 monoclonal antibody (Product # 48-7069-42) in T-Cell Activation

Thu Dec 14 00:00:00 EST 2017

Mesenchymal Stromal Cell-Derived Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Acquisition of Epithelial Stem-Like Cell Properties in Ameloblastoma Epithelial Cells.

Stem cells (Dayton, Ohio)

Jiang C,Zhang Q,Shanti RM,Shi S,Chang TH,Carrasco L,Alawi F,Le AD

Published figure using IL-6 monoclonal antibody (Product # 48-7069-42) in Immunofluorescence

Fri Sep 01 00:00:00 EDT 2017

Biallelic hypomorphic mutations in a linear deubiquitinase define otulipenia, an early-onset autoinflammatory disease.

Proceedings of the National Academy of Sciences of the United States of America

Zhou Q,Yu X,Demirkaya E,Deuitch N,Stone D,Tsai WL,Kuehn HS,Wang H,Yang D,Park YH,Ombrello AK,Blake M,Romeo T,Remmers EF,Chae JJ,Mullikin JC,Güzel F,Milner JD,Boehm M,Rosenzweig SD,Gadina M,Welch SB,Özen S,Topaloglu R,Abinun M,Kastner DL,Aksentijevich I

Published figure using IL-6 monoclonal antibody (Product # 48-7069-42) in ELISA

Tue Sep 06 00:00:00 EDT 2016

Biallelic hypomorphic mutations in a linear deubiquitinase define otulipenia, an early-onset autoinflammatory disease.

Proceedings of the National Academy of Sciences of the United States of America

Published figure using IL-6 monoclonal antibody (Product # 48-7069-42) in ELISA

Tue Sep 06 00:00:00 EDT 2016

TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.

PloS one

Reichwald K,Jørgensen TZ,Tougaard P,Skov S

Published figure using IL-6 monoclonal antibody (Product # 48-7069-42) in Flow Cytometry

Mon Sep 08 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

UniProt ID: (Human) P05231

Entrez Gene ID: (Human) 3569

Function(s)

Process(es)

cytokine activity interleukin-6 receptor binding protein binding growth factor activity

IL-6 Monoclonal Antibody (MQ2-13A5), eFluor™ 450, eBioscience™

Product Details

48-7069-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

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