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Bethyl Laboratories
IMPACT Polyclonal Antibody
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FIGURE: 1 / 1
IMPACT Antibody (A302-661A) in WB
Detection of human IMPACT by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-IMPACT antibody (Product # A302-661A) used for WB at 0.4 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. IMPACT was also immunoprecipitated by rabbit anti-IMPACT antibody BL9612, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Product Details
A302-661A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 270 and 320 of human imprinted and ancient homolog.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, plays a role in differentiation of neuronal cells by stimulating neurite outgrowth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Impact homolog; Imprinted and ancient gene protein homolog; Protein IMPACT; RWD domain containing 5
Gene Aliases: IMPACT; RWDD5
UniProt ID: (Human) Q9P2X3
Entrez Gene ID: (Human) 55364
negative regulation of transcription from RNA polymerase II promoter
negative regulation of protein phosphorylation
biological_process
negative regulation of protein complex assembly
negative regulation of protein autophosphorylation
cellular response to amino acid starvation
cellular response to glucose starvation
positive regulation of neuron differentiation
negative regulation of cell death
regulation of eIF2 alpha phosphorylation by amino acid starvation
cellular response to hydrogen peroxide
positive regulation of translational initiation in response to starvation
cellular response to acidic pH
cellular response to UV-C
cellular response to benomyl
negative regulation of transcription from RNA polymerase II promoter in response to stress
neuron projection extension
cellular response to leucine starvation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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