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IMPDH2 Polyclonal Antibody

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IMPDH2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using IMPDH2 antibody 12948-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

IMPDH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using 12948-1-AP (IMPDH2 antibody) at dilution of 1:100 (under 10x lens).

IMPDH2 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 12948-1-AP (IMPDH2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

IMPDH2 Antibody in Immunoprecipitation (IP) — IP result of anti-IMPDH2 (IP:12948-1-AP, 3ug; Detection:12948-1-AP 1:800) with K-562 cells lysate 4000ug.

Product Details

12948-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,200	View 7 publications 7 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 11 publications 11 publications
ELISA (ELISA)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IMPDH2 Fusion Protein Ag3632 (1-350 aa encoded by BC012840) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.26 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MADYLISGG TSYVPDDGLT AQQLFNCGDG LTYNDFLILP GYIDFTADQV DLTSALTKKI TLKTPLVSSP MDTVTEAGMA IAMALTGGIG FIHHNCTPEF QANEVRKVKK YEQGFITDPV VLSPKDRVRD VFEAKARHGF CGIPITDTGR MGSRLVGIIS SRDIDFLKEE EHDCFLEEIM TKREDLVVAP AGITLKEANE ILQRSKKGKL PIVNEDDELV AIIARTDLKK NRDYPLASKD AKKQLLCGAA IGTHEDDKYR LDLLAQAGVD VVVLDSSQGN SIFQINMIKY IKDKYPNLQV IGGNVVTAAQ AKNLIDAGVD ALRVGMGSGS ICITQEVLAC GRPQATAVYK V (1-350 aa encoded by BC012840)

Target Information

This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

IMP/GTP balance modulates cytoophidium assembly and IMPDH activity.

Cell division

Keppeke GD,Chang CC,Peng M,Chen LY,Lin WC,Pai LM,Andrade LEC,Sung LY,Liu JL

Fri Mar 18 00:00:00 EDT 2022

IMP/GTP balance modulates cytoophidium assembly and IMPDH activity.

Cell division

Keppeke GD,Chang CC,Peng M,Chen LY,Lin WC,Pai LM,Andrade LEC,Sung LY,Liu JL

Fri Mar 18 00:00:00 EDT 2022

Immune Response-Dependent Assembly of IMP Dehydrogenase Filaments.

Frontiers in immunology

Calise SJ,Abboud G,Kasahara H,Morel L,Chan EKL

Thu Oct 03 00:00:00 EDT 2019

Ribavirin induces widespread accumulation of IMP dehydrogenase into rods/rings structures in multiple major mouse organs.

Antiviral research

Keppeke GD,Calise SJ,Chan EKL,Andrade LEC

Fri Feb 01 00:00:00 EST 2019

Interfilament interaction between IMPDH and CTPS cytoophidia.

The FEBS journal

Chang CC,Keppeke GD,Sung LY,Liu JL

Mon Oct 01 00:00:00 EDT 2018

Interfilament interaction between IMPDH and CTPS cytoophidia.

The FEBS journal

Chang CC,Keppeke GD,Sung LY,Liu JL

Mon Oct 01 00:00:00 EDT 2018

Differential capacity of therapeutic drugs to induce Rods/Rings structures in vitro and in vivo and generation of anti-Rods/Rings autoantibodies.

Clinical immunology (Orlando, Fla.)

Keppeke GD,Prado MS,Nunes E,Perazzio SF,Rodrigues SH,Ferraz ML,Chan EK,Andrade LE

Thu Dec 01 00:00:00 EST 2016

'Rod and ring' formation from IMP dehydrogenase is regulated through the one-carbon metabolic pathway.

Journal of cell science

Calise SJ,Purich DL,Nguyen T,Saleem DA,Krueger C,Yin JD,Chan EK

Mon Aug 01 00:00:00 EDT 2016

Assembly of IMPDH2-based, CTPS-based, and mixed rod/ring structures is dependent on cell type and conditions of induction.

Journal of genetics and genomics = Yi chuan xue bao

Keppeke GD,Calise SJ,Chan EK,Andrade LE

Sat Jun 20 00:00:00 EDT 2015

Microinjection of specific anti-IMPDH2 antibodies induces disassembly of cytoplasmic rods/rings that are primarily stationary and stable structures.

Cell & bioscience

Keppeke GD,Andrade LE,Grieshaber SS,Chan EK

Tue Jan 20 00:00:00 EST 2015

Temporal evolution of human autoantibody response to cytoplasmic rods and rings structure during anti-HCV therapy with ribavirin and interferon-α.

Immunologic research

Keppeke GD,Satoh M,Ferraz ML,Chan EK,Andrade LE

Wed Oct 01 00:00:00 EDT 2014

Ultrastructure of cytoplasmic and nuclear inosine-5'-monophosphate dehydrogenase 2 "rods and rings" inclusions.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Juda P,Smigová J,Kováčik L,Bártová E,Raška I

Wed Oct 01 00:00:00 EDT 2014

Ultrastructure of cytoplasmic and nuclear inosine-5'-monophosphate dehydrogenase 2 "rods and rings" inclusions.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Juda P,Smigová J,Kováčik L,Bártová E,Raška I

Wed Oct 01 00:00:00 EDT 2014

Glutamine deprivation initiates reversible assembly of mammalian rods and rings.

Cellular and molecular life sciences : CMLS

Calise SJ,Carcamo WC,Krueger C,Yin JD,Purich DL,Chan EK

Fri Aug 01 00:00:00 EDT 2014

Glutamine deprivation initiates reversible assembly of mammalian rods and rings.

Cellular and molecular life sciences : CMLS

Calise SJ,Carcamo WC,Krueger C,Yin JD,Purich DL,Chan EK

Fri Aug 01 00:00:00 EDT 2014

A simple Fourier filter for suppression of the missing wedge ray artefacts in single-axis electron tomographic reconstructions.

Journal of structural biology

Kováčik L,Kereïche S,Kerïeche S,Höög JL,Jůda P,Matula P,Raška I

Tue Apr 01 00:00:00 EDT 2014

Hirschsprung-like disease is exacerbated by reduced de novo GMP synthesis.

The Journal of clinical investigation

Lake JI,Tusheva OA,Graham BL,Heuckeroth RO

Fri Nov 01 00:00:00 EDT 2013

Induction of cytoplasmic rods and rings structures by inhibition of the CTP and GTP synthetic pathway in mammalian cells.

PloS one

Carcamo WC,Satoh M,Kasahara H,Terada N,Hamazaki T,Chan JY,Yao B,Tamayo S,Covini G,von Mühlen CA,Chan EK

Wed Apr 25 00:00:00 EDT 2012

Induction of cytoplasmic rods and rings structures by inhibition of the CTP and GTP synthetic pathway in mammalian cells.

PloS one

Carcamo WC,Satoh M,Kasahara H,Terada N,Hamazaki T,Chan JY,Yao B,Tamayo S,Covini G,von Mühlen CA,Chan EK

Wed Apr 25 00:00:00 EDT 2012

Identification of IMPDH2 as a tumor-associated antigen in colorectal cancer using immunoproteomics analysis.

International journal of colorectal disease

He Y,Mou Z,Li W,Liu B,Fu T,Zhao S,Xiang D,Wu Y

Sun Nov 01 00:00:00 EDT 2009

Characterisation of inosine monophosphate dehydrogenase expression during retinal development: differences between variants and isoforms.

The international journal of biochemistry & cell biology

Gunter JH,Thomas EC,Lengefeld N,Kruger SJ,Worton L,Gardiner EM,Jones A,Barnett NL,Whitehead JP

Tue Sep 30 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: IMP (inosine 5'-monophosphate) dehydrogenase 2; IMP (inosine monophosphate) dehydrogenase 2; IMP dehydrogenase 2; IMP dehydrogenase 2 {ECO:0000255|HAMAP-Rule:MF_03156}; IMP dehydrogenase II; IMP dehydrogenase type II; IMP oxireductase 2; IMPD 2; IMPD 2 {ECO:0000255|HAMAP-Rule:MF_03156}; IMPDH 2; IMPDH 2 {ECO:0000255|HAMAP-Rule:MF_03156}; IMPDH II; IMPDH-II; IMPDH2; inosine 5' phosphate dehydrogenase 2; inosine 5'-phosphate dehydrogenase 2; inosine 5-monophosphate dehydrogenase 2; inosine monophosphate dehydrogenase 2; inosine monophosphate dehydrogenase type II; Inosine-5'-monophosphate dehydrogenase 2; inosine-5'-monophosphate dehydrogenase 2 {ECO:0000255|HAMAP-Rule:MF_03156}; Inosine-5'-monophosphate dehydrogenase type II

Gene Aliases: IMPD; IMPD 2; IMPD2; IMPDH 2; IMPDH-II; IMPDH2

UniProt ID: (Human) P12268, (Mouse) P24547

Entrez Gene ID: (Human) 3615, (Rat) 301005, (Mouse) 23918

Molecular Function: dehydrogenase oxidoreductase metabolite interconversion enzyme