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          • Primary Antibodies ›
          • mCherry Antibodies

          Invitrogen

          mCherry Monoclonal Antibody (16D7), Alexa Fluor™ 647

          3 References
          View all (39) mCherry antibodies

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          Cite mCherry Monoclonal Antibody (16D7), Alexa Fluor™ 647

          Additional Information:
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          • Antibody Testing Data (3)
          mCherry Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          mCherry Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          mCherry Antibody (M11241) in ICC/IF

          Immunofluorescent analysis of mCherry Tag was performed using 70% confluent mCherry-H3 transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with mCherry Monoclonal Antibody (16D7), Alexa Fluor 647 (Product # M11241) at 1:100 dilution and Histone H3 Recombinant Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then l... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          mCherry Antibody in Immunocytochemistry (ICC/IF)
          mCherry Antibody in Western Blot (WB)
          mCherry Antibody in Flow Cytometry (Flow)

          Product Details

          M11241

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunocytochemistry (ICC/IF)

          1:100
          -

          Flow Cytometry (Flow)

          Assay-dependent
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Chemical

          Published species

          Tag

          Host/Isotype

          Rat / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          16D7

          Immunogen

          Full-length mCherry was used as the immunogen

          Conjugate

          Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647
          • Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 594 
          • Pacific Blue
          • Request custom conjugation

          Excitation/Emission Max

          650/671 nm View spectra spectra

          Form

          Liquid

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.5

          Contains

          5mM sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2536615

          Target Information

          MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components. mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t 0. 5 of 15 minutes, allowing it to be visualized soon after translation.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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