Search
Invitrogen
SOX2 Monoclonal Antibody (20G5)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
{{$ctrl.translations['antibody.pdp.skuNotes.additionalInformation']}}:
FIGURE: 1 / 35
SOX2 Antibody (MA1-014) in ICC/IF
Immunofluorescent analysis of Sox2 (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Sox2 monoclonal antibody (Product # MA1-014) at a dilution of 1:200 overnight at 4°C, washed with PBST, and incubated with a FITC-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fl... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-014
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Fish,
Human,
Mouse
Published species
Bovine,
Dog,
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
20G5
Immunogen
Full-length human recombinant protein expressed in bacteria
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 30% glycerol, 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Western blot analysis of MA1-014 specifically detects SOX2 protein at ~36 kDa in the nucleus of human and mouse embryonal carcinoma and iPS cells.
Target Information
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); putative; sex determining region Y-box 2; SOX 2; sox-2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY related HMG box 2; SRY-box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; transcription factor SOX2; unnamed protein product
Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; ysb
UniProt ID: (Human) P48431, (Mouse) P48432
Entrez Gene ID: (Dog) 488092, (Human) 6657, (Mouse) 20674
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
miRNA binding
sequence-specific DNA binding
chromatin binding
chromatin DNA binding
nitric-oxide synthase binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
DNA-binding transcription factor binding
HMG box transcription factor
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
eye development
endodermal cell fate specification
chromatin organization
regulation of transcription, DNA-templated
brain development
response to wounding
regulation of gene expression
glial cell fate commitment
pituitary gland development
neuron differentiation
forebrain development
somatic stem cell population maintenance
positive regulation of MAPK cascade
negative regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
inner ear development
response to growth factor
negative regulation of canonical Wnt signaling pathway
neuronal stem cell population maintenance
negative regulation of cell cycle G1/S phase transition
cell fate specification
positive regulation of neuroblast proliferation
regulation of transcription from RNA polymerase II promoter
sensory perception of sound
gene expression
stem cell population maintenance
forebrain neuron differentiation
adenohypophysis development
cerebral cortex development
positive regulation of cell-cell adhesion
negative regulation of Wnt signaling pathway
male genitalia development
positive regulation of epithelial cell differentiation
olfactory placode formation
response to retinoic acid
inner ear morphogenesis
tongue development
cell fate commitment
negative regulation of cell differentiation
positive regulation of cell differentiation
positive regulation of neuron differentiation
negative regulation of osteoblast differentiation
positive regulation of Notch signaling pathway
pigment biosynthetic process
lung alveolus development
embryonic organ development
anatomical structure formation involved in morphogenesis
neuron fate commitment
diencephalon morphogenesis
stem cell differentiation
regulation of neurogenesis
detection of mechanical stimulus involved in sensory perception of sound
detection of mechanical stimulus involved in equilibrioception
retina morphogenesis in camera-type eye
lens induction in camera-type eye
epithelial tube branching involved in lung morphogenesis
cellular response to cadmium ion
response to oxygen-glucose deprivation
regulation of myofibroblast cell apoptotic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support