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Invitrogen
SOX2 Monoclonal Antibody (20G5), DyLight™ 650
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FIGURE: 1 / 5
SOX2 Antibody (MA1-014-D650) in ICC/IF
Immunofluorescent analysis of SOX2 (red) showing nuclear staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 650-conjugated SOX2 monoclonal antibody (Product # MA1-014-D650) in 3% BSA-PBS at a dilution of 1:50 and incubated for 1 hour at 37C in the dark. Nuclei (left panel, blue) were stained with D... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-014-D650
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
20G5
Immunogen
Full-length human recombinant protein expressed in bacteria
Conjugate
DyLight™ 650
DyLight™ 650
DyLight™ 650
Excitation/Emission Max
651/673 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with proprietary stabilizer
Contains
0.02% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-014-D650 has been successfully used in ICC/IF applications with human and mouse samples.
MA1-014-D650 can be used for immunofluorescence analysis of LIN28 in human iPSCs.
Target Information
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Delta EF2a; MCOPS3 (Microphthalmia Syndromic type 3); putative; sex determining region Y-box 2; SOX 2; sox-2; SRY (sex determining region Y) box 2; SRY (sex determining region Y)-box 2; SRY related HMG box 2; SRY-box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; transcription factor SOX2; unnamed protein product
Gene Aliases: ANOP3; lcc; MCOPS3; Sox-2; ysb
UniProt ID: (Human) P48431, (Mouse) P48432
Entrez Gene ID: (Human) 6657, (Mouse) 20674
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
miRNA binding
sequence-specific DNA binding
chromatin binding
chromatin DNA binding
nitric-oxide synthase binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
DNA-binding transcription factor binding
negative regulation of transcription from RNA polymerase II promoter
osteoblast differentiation
cell fate specification
endodermal cell fate specification
positive regulation of neuroblast proliferation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
brain development
sensory perception of sound
gene expression
regulation of gene expression
stem cell population maintenance
forebrain neuron differentiation
adenohypophysis development
cerebral cortex development
positive regulation of cell-cell adhesion
negative regulation of Wnt signaling pathway
neuron differentiation
male genitalia development
positive regulation of epithelial cell differentiation
forebrain development
olfactory placode formation
response to retinoic acid
somatic stem cell population maintenance
inner ear morphogenesis
positive regulation of MAPK cascade
tongue development
cell fate commitment
negative regulation of cell differentiation
positive regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of neuron differentiation
negative regulation of osteoblast differentiation
positive regulation of Notch signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
pigment biosynthetic process
lung alveolus development
embryonic organ development
anatomical structure formation involved in morphogenesis
neuron fate commitment
inner ear development
diencephalon morphogenesis
stem cell differentiation
regulation of neurogenesis
detection of mechanical stimulus involved in sensory perception of sound
detection of mechanical stimulus involved in equilibrioception
retina morphogenesis in camera-type eye
lens induction in camera-type eye
epithelial tube branching involved in lung morphogenesis
response to growth factor
cellular response to cadmium ion
negative regulation of canonical Wnt signaling pathway
response to oxygen-glucose deprivation
neuronal stem cell population maintenance
negative regulation of cell cycle G1/S phase transition
regulation of myofibroblast cell apoptotic process
eye development
chromatin organization
response to wounding
glial cell fate commitment
pituitary gland development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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