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Invitrogen
CD11b Monoclonal Antibody (M1/70)
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FIGURE: 1 / 2
CD11b Antibody (MA1-10080) in Flow
Separation of murine CD11b positive myeloid cells (red-filled) from murine CD11b negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD11b (M1/70) purified Monoclonal antibody (Product # MA1-10080). Low endotoxin, concentration in sample 0.6 µg/mL, DAR APC.
Product Details
MA1-10080
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rabbit
Published species
Human,
Mouse,
Rabbit
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
M1/70
Immunogen
B10 mouse spleen cells enriched for T cells
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects an extracellular epitope of CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
Functional Application: Blocking.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; CD11B (p170); cell surface glycoprotein MAC-1 alpha subunit; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; complement component receptor 3 alpha-a; complement receptor type 3; CR-3 alpha chain; glycoprotein Mac-1; Integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); leukocyte adhesion glycoprotein precursor; Leukocyte adhesion receptor MO1; Mac-1 alpha; Mac-1 alpha (Mac1A); macrophage antigen alpha; macrophage antigen alpha polypeptide; macrophage-1 antigen alpha subunit; MGC117044; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; unnamed protein product
Gene Aliases: CD11B; CD11b/CD18; CR3; CR3A; F730045J24Rik; ITGAM; Ly-40; MAC-1; Mac-1a; MAC1; MAC1A; MO1A; SLEB6
UniProt ID: (Human) Q4VAK1
Entrez Gene ID: (Human) 3684, (Mouse) 16409, (Rabbit) 100351865
beta-amyloid binding
complement component C3b binding
integrin binding
protein binding
heat shock protein binding
signaling receptor activity
cargo receptor activity
macromolecular complex binding
metal ion binding
opsonin binding
glycoprotein binding
heparin binding
heparan sulfate proteoglycan binding
protein heterodimerization activity
integrin
microglial cell activation
immune system process
complement receptor mediated signaling pathway
response to ischemia
receptor-mediated endocytosis
phagocytosis, engulfment
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
response to mechanical stimulus
positive regulation of metabolic process
ectodermal cell differentiation
forebrain development
response to estradiol
positive regulation of superoxide anion generation
heterotypic cell-cell adhesion
positive regulation of neutrophil degranulation
innate immune response
negative regulation of dopamine metabolic process
positive regulation of protein targeting to membrane
beta-amyloid clearance
cell-cell adhesion
cell-cell adhesion via plasma-membrane adhesion molecules
response to Gram-positive bacterium
complement-mediated synapse pruning
vertebrate eye-specific patterning
positive regulation of microglial cell mediated cytotoxicity
response to curcumin
leukocyte migration involved in inflammatory response
leukocyte cell-cell adhesion
microglia development
single organismal cell-cell adhesion
neutrophil chemotaxis
cellular extravasation
activated T cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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