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Invitrogen
HDAC1 Monoclonal Antibody (10E2)
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FIGURE: 1 / 6
HDAC1 Antibody (MA5-18071) in ICC/IF
Immunofluorescence analysis of HDAC1 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HDAC1 Monoclonal Antibody (10E2) (Product # MA5-18071) at 1 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A28175) at a... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-18071
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
10E2
Immunogen
Synthetic peptide: EEKPEAKGVKEEVKLA, corresponding to C terminal amino acids 467-482 of Human and mouse HDAC1
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This target has a predicted molecular weight of 55 kDa. Actual Western blot results show a band at 57 kDa.
Target Information
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp686H12203; HD1; Histone deacetylase 1; Protein deacetylase HDAC1; Protein deacylase HDAC1; RP4-811H24.2
Gene Aliases: HD1; HDAC1; Hdac1-ps; MommeD5; RPD3; RPD3L1
UniProt ID: (Mouse) O09106, (Rat) Q4QQW4
Entrez Gene ID: (Mouse) 433759, (Rat) 297893
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
core promoter binding
RNA polymerase II repressing transcription factor binding
RNA polymerase II transcription corepressor activity
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
histone deacetylase activity
protein binding
transcription factor binding
hydrolase activity
deacetylase activity
enzyme binding
histone deacetylase activity (H3-K14 specific)
nucleosomal DNA binding
NAD-dependent histone deacetylase activity (H3-K14 specific)
histone deacetylase activity (H3-K9 specific)
protein complex binding
protein deacetylase activity
activating transcription factor binding
histone deacetylase activity (H4-K16 specific)
Krueppel-associated box domain binding
histone deacetylase binding
transcription regulatory region DNA binding
NAD-dependent histone deacetylase activity (H3-K9 specific)
NAD-dependent histone deacetylase activity (H4-K16 specific)
protein N-terminus binding
NF-kappaB binding
repressing transcription factor binding
NAD-dependent histone deacetylase activity (H3-K18 specific)
negative regulation of transcription from RNA polymerase II promoter
response to amphetamine
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
protein deacetylation
endoderm development
circadian rhythm
positive regulation of cell proliferation
negative regulation of cell proliferation
epidermal cell differentiation
negative regulation of gene expression
positive regulation of receptor biosynthetic process
chromatin modification
histone deacetylation
hippocampus development
neuron differentiation
positive regulation of interleukin-1 production
positive regulation of tumor necrosis factor production
circadian regulation of gene expression
cellular response to oxidative stress
odontogenesis of dentin-containing tooth
positive regulation of tyrosine phosphorylation of Stat3 protein
embryonic digit morphogenesis
ATP-dependent chromatin remodeling
negative regulation of apoptotic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of neuron apoptotic process
negative regulation by host of viral transcription
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin secretion
rhythmic process
positive regulation of oligodendrocyte differentiation
negative regulation of androgen receptor signaling pathway
hair follicle placode formation
eyelid development in camera-type eye
fungiform papilla formation
histone H3 deacetylation
histone H4 deacetylation
negative regulation of canonical Wnt signaling pathway
positive regulation of chemokine (C-X-C motif) ligand 2 production
positive regulation of type B pancreatic cell apoptotic process
negative regulation of peptidyl-lysine acetylation
negative regulation of intrinsic apoptotic signaling pathway
negative regulation of myotube differentiation
response to caffeine
response to lipopolysaccharide
response to drug
response to hyperoxia
cellular response to tumor necrosis factor
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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