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Invitrogen
NMDAR2A Monoclonal Antibody (N327/95)
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FIGURE: 1 / 2
NMDAR2A Antibody (MA5-27692) in ICC/IF
Immunofluorescent analysis of NMDAR2A in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with NMDAR2A monoclonal antibody (Product # MA5-27692) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) GluN2A/NR2A Antibody, D) Merged image. Mag... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-27692
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
N327/95
Immunogen
Fusion protein amino acids 75-325 (extracellular N-terminus) of rat GluN2A/NR2A
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-27692 was sufficient for detection o GluN2A/NR2A in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 170kDa. Does not react with NR2B.
This antibody was formerly sold as clone S327-95.
Target Information
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: glutamate [NMDA] receptor subunit epsilon-1; glutamate ionotropic receptor NMDA 2A; glutamate receptor; glutamate receptor, ionotropic, N-methyl D-aspartate 2A; hNR2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor channel, subunit epsilon-1; N-methyl-D-aspartate receptor subunit 2A; NMDA Receptor 2A; NMDAR 2A; OTTHUMP00000160135; OTTHUMP00000174531; unnamed protein product
Gene Aliases: EPND; FESD; GluN2A; GluRepsilon1; LKS; NMDAR2A; NR2A
UniProt ID: (Human) Q12879, (Rat) Q00959, (Mouse) P35436
Entrez Gene ID: (Human) 2903, (Rat) 24409, (Mouse) 14811
beta-amyloid binding
NMDA glutamate receptor activity
ion channel activity
cation channel activity
calcium channel activity
protein binding
zinc ion binding
ligand-gated ion channel activity
glutamate-gated calcium ion channel activity
signaling receptor activity
transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential
ionotropic glutamate receptor activity
receptor binding
extracellular-glutamate-gated ion channel activity
glutamate binding
protein kinase binding
voltage-gated cation channel activity
glutamate receptor binding
macromolecular complex binding
calcium-dependent protein binding
cell adhesion molecule binding
ATPase binding
scaffold protein binding
ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential
protein tyrosine kinase binding
action potential
receptor recycling
startle response
response to amphetamine
ion transport
calcium ion transport
positive regulation of cytosolic calcium ion concentration
glutamate receptor signaling pathway
chemical synaptic transmission
learning or memory
learning
memory
associative learning
visual learning
response to xenobiotic stimulus
response to light stimulus
response to wounding
response to carbohydrate
response to manganese ion
response to lithium ion
response to lead ion
response to amine
sensory perception of pain
calcium-mediated signaling
spinal cord development
mesencephalic trigeminal nucleus development
hippocampus development
cerebral cortex development
neurogenesis
sleep
auditory behavior
regulation of ARF protein signal transduction
directional locomotion
multicellular organismal response to stress
response to nicotine
ionotropic glutamate receptor signaling pathway
synaptic transmission, glutamatergic
sodium ion transmembrane transport
locomotion
negative regulation of protein catabolic process
response to cocaine
regulation of membrane potential
dopamine metabolic process
serotonin metabolic process
positive regulation of apoptotic process
response to ethanol
regulation of synaptic plasticity
regulation of neuronal synaptic plasticity
regulation of long-term neuronal synaptic plasticity
modulation of synaptic transmission
positive regulation of mitochondrial calcium ion concentration
response to calcium ion
response to methylmercury
response to other organism
positive regulation of synaptic transmission, glutamatergic
regulation of postsynaptic membrane potential
excitatory postsynaptic potential
long-term synaptic potentiation
response to ammonium ion
response to fungicide
calcium ion transmembrane transport
cellular response to amino acid stimulus
cellular response to magnesium ion
cellular response to manganese ion
cellular response to zinc ion
cellular response to dsRNA
cellular response to growth factor stimulus
cellular response to lipid
positive regulation of protein targeting to membrane
response to environmental enrichment
dendritic spine organization
positive regulation of inhibitory postsynaptic potential
calcium ion transmembrane import into cytosol
cation transmembrane transport
modulation of excitatory postsynaptic potential
regulation of presynaptic membrane potential
neurotransmitter receptor transport, plasma membrane to endosome
regulation of response to alcohol
response to glycoside
protein localization to postsynaptic membrane
regulation of cation transmembrane transport
cellular response to beta-amyloid
response to hydrogen sulfide
cellular response to glycine
conditioned place preference
regulation of N-methyl-D-aspartate selective glutamate receptor activity
positive regulation of excitatory postsynaptic potential
brain development
excitatory chemical synaptic transmission
locomotory behavior
rhythmic process
neuromuscular process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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