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          • Primary Antibodies ›
          • ZAP70/Syk Antibodies

          Invitrogen

          Phospho-ZAP70/Syk (Tyr319, Tyr352) Recombinant Rabbit Monoclonal Antibody (Zap70Y319-A3)

          View all (11) ZAP70/Syk antibodies

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          Cite Phospho-ZAP70/Syk (Tyr319, Tyr352) Recombinant Rabbit Monoclonal Antibody (Zap70Y319-A3)

          Additional Information:
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          • Antibody Testing Data (4)
          Phospho-ZAP70/Syk (Tyr319, Tyr352) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          Phospho-ZAP70/Syk (Tyr319, Tyr352) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          Phospho-ZAP70/Syk (Tyr319, Tyr352) Antibody (MA5-36961) in Flow

          Flow cytometry of Phospho-Zap70 (Tyr319, Tyr352) using Jurkat cells. Samples were incubated in monoclonal Phospho-Zap70 (Tyr319, Tyr352) antibody (Product # MA5-36961) with a dilution of 0.05 µg/mL. Images correspond with the following conditions: negative control (blue) untreated (red) treated with pervanadate (green) treated/blocked with non-phospho-peptide (violet), treated /blocked with phospho-peptide ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Phospho-ZAP70/Syk (Tyr319, Tyr352) Antibody in Flow Cytometry (Flow)
          Phospho-ZAP70/Syk (Tyr319, Tyr352) Antibody in Flow Cytometry (Flow)
          Phospho-ZAP70/Syk (Tyr319, Tyr352) Antibody in Flow Cytometry (Flow)
          Phospho-ZAP70/Syk (Tyr319, Tyr352) Antibody in Flow Cytometry (Flow)

          Product Details

          MA5-36961

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          1-1,000 ng/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG, kappa

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          Zap70Y319-A3

          Immunogen

          A synthetic phospho-peptide corresponding to residues surrounding Tyr319/Tyr352 of human phospho Zap70/Syk.

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • APC 
          • FITC
          • PE 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Amount

          10 µg

          Purification

          Protein A/G

          Storage buffer

          PBS, pH 7.4, with 50% glycerol, 0.1% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2896896

          Product Specific Information

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          Target Information

          ZAP70 and SYK are closely related cytoplasmic tyrosine kinases that play central roles in immune receptor signaling. ZAP70 (Zeta-chain-associated protein kinase 70; gene: ZAP70) is primarily expressed in T lymphocytes and natural killer (NK) cells, where it is recruited to the T cell receptor (TCR) complex following antigen recognition. Upon activation, ZAP70 phosphorylates downstream adaptor proteins and initiates signaling cascades that drive T cell activation, proliferation, and differentiation. SYK (Spleen Tyrosine Kinase; gene: SYK) is predominantly expressed in B cells and other hematopoietic cells and functions analogously in B cell receptor (BCR) signaling, as well as in Fc receptor and integrin-mediated pathways. Although ZAP70 and SYK share structural homology and overlapping signaling mechanisms, they exhibit distinct expression patterns and immunological roles. Dysregulation of either kinase has been implicated in autoimmune disease, immunodeficiency, and hematologic malignancies, making them important targets in immunology and therapeutic research.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; p72-Syk; Spleen tyrosine kinase; Syk kinase; Syk-related kinase; Syk-related tyrosine kinase; TCR-associated protein tyrosine kinase; Tyrosine-protein kinase SYK; Tyrosine-protein kinase ZAP-70; unnamed protein product; ZAP-70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD

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          Gene Aliases: ADMIO2; IMD48; IMD82; mrtle; mur; p72-Syk; ptk72; SRK; STCD; STD; SYK; Sykb; TZK; ZAP-70; ZAP70

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          UniProt ID: (Human) P43405, (Mouse) P48025, (Human) P43403, (Mouse) P43404

          View more View less

          Entrez Gene ID: (Human) 6850, (Mouse) 20963, (Human) 7535, (Mouse) 22637

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          Function(s)
          nucleotide binding phosphotyrosine binding protein kinase activity protein serine/threonine kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding integrin binding protein binding ATP binding interleukin-15 receptor binding kinase activity transferase activity protein kinase binding phosphatase binding Toll-like receptor binding SH2 domain binding phospholipase binding scaffold protein binding receptor signaling protein tyrosine kinase activity transferase activity, transferring phosphorus-containing groups protein domain specific binding kinase modulator
          Process(es)
          autophagosome assembly angiogenesis cell activation positive regulation of cytokine production serotonin secretion lymph vessel development positive regulation of receptor internalization stimulatory C-type lectin receptor signaling pathway adaptive immune response macrophage activation involved in immune response neutrophil activation involved in immune response leukocyte activation involved in immune response immune system process serotonin secretion by platelet cell surface pattern recognition receptor signaling pathway negative regulation of inflammatory response to antigenic stimulus protein phosphorylation protein import into nucleus leukocyte cell-cell adhesion cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway integrin-mediated signaling pathway organ morphogenesis negative regulation of autophagy positive regulation of autophagy regulation of platelet activation regulation of tumor necrosis factor-mediated signaling pathway peptidyl-tyrosine phosphorylation leukotriene biosynthetic process calcium-mediated signaling platelet activation B cell differentiation neutrophil chemotaxis positive regulation of protein complex assembly receptor internalization cellular response to nutrient levels positive regulation of type I interferon production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-3 production positive regulation of interleukin-4 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of mast cell cytokine production regulation of superoxide anion generation positive regulation of superoxide anion generation positive regulation of cell adhesion mediated by integrin intracellular signal transduction collagen-activated tyrosine kinase receptor signaling pathway Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis interleukin-3-mediated signaling pathway TORC1 signaling gamma-delta T cell differentiation defense response to bacterium mast cell degranulation positive regulation of mast cell degranulation regulation of neutrophil degranulation beta selection positive regulation of MAPK cascade innate immune response positive regulation of B cell differentiation positive regulation of gamma-delta T cell differentiation positive regulation of lymphocyte differentiation positive regulation of bone resorption positive regulation of alpha-beta T cell differentiation positive regulation of alpha-beta T cell proliferation blood vessel morphogenesis regulation of phagocytosis regulation of immune response positive regulation of calcium-mediated signaling B cell receptor signaling pathway leukocyte migration regulation of sequence-specific DNA binding transcription factor activity establishment of localization in cell regulation of ERK1 and ERK2 cascade cellular response to molecule of fungal origin cellular response to lipid cellular response to low-density lipoprotein particle stimulus positive regulation of monocyte chemotactic protein-1 production regulation of arachidonic acid secretion regulation of platelet aggregation apoptotic signaling pathway beta-amyloid clearance positive regulation of cold-induced thermogenesis negative regulation of TORC1 signaling positive regulation of TORC1 signaling cellular response to beta-amyloid cellular response to lectin activation of MAPK activity transmembrane receptor protein tyrosine kinase signaling pathway G-protein coupled receptor signaling pathway activation of JUN kinase activity phosphorylation cell migration peptidyl-serine phosphorylation signal transduction by protein phosphorylation cell differentiation peptidyl-tyrosine autophosphorylation transcription factor import into nucleus positive regulation of interleukin-3 biosynthetic process positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process protein autophosphorylation positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation immune response T cell differentiation T cell activation B cell activation positive thymic T cell selection negative thymic T cell selection thymic T cell selection positive regulation of T cell differentiation alpha-beta T cell differentiation T cell receptor signaling pathway T cell aggregation T cell migration
          It has to be done as per old AB suggested Products section.

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