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Invitrogen
VE-cadherin Monoclonal Antibody (55-7H1), Alexa Fluor™ 647
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FIGURE: 1 / 1
VE-cadherin Antibody (MA5-44146) in Flow
Separation of HUVEC cells (red-filled) from HL-60 cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD144 (55-7H1) Alexa Fluor® 647 Monoclonal antibody (Product # MA5-44146), 4 µL reagent per milion cells in 100 µL of cell suspension.
Product Details
MA5-44146
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
55-7H1
Immunogen
Human endothelial cells
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes a calcium-independent extracellular epitope on CD144 (VE-cadherin, cadherin 5), an adhesion molecule expressed on endothelial cells.
Target Information
VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 7B4 antigen; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144; cd144 antigen; endothelial-specific cadherin; unnamed protein product; Vascular endothelial cadherin; VE-cadherin
Gene Aliases: 7B4; CD144; CDH5
UniProt ID: (Human) P33151
Entrez Gene ID: (Human) 1003
receptor binding
calcium ion binding
protein binding
beta-catenin binding
protein phosphatase binding
receptor signaling complex scaffold activity
vascular endothelial growth factor receptor 2 binding
ion channel binding
cadherin binding
metal ion binding
fibrinogen binding
BMP receptor binding
protein binding involved in cell-cell adhesion
protein tyrosine kinase binding
cell morphogenesis
endothelial cell morphogenesis
blood vessel maturation
cell migration involved in sprouting angiogenesis
cellular calcium ion homeostasis
cell-cell junction assembly
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
cell surface receptor signaling pathway
transforming growth factor beta receptor signaling pathway
negative regulation of cell proliferation
positive regulation of gene expression
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
positive regulation of cell migration
positive regulation of BMP signaling pathway
negative regulation of microtubule polymerization
positive regulation of protein complex assembly
adherens junction organization
maintenance of blood-brain barrier
regulation of vascular permeability
blood vessel endothelial cell migration
cell-cell adhesion mediated by cadherin
positive regulation of angiogenesis
negative regulation of inflammatory response
bicellular tight junction assembly
cell-cell adhesion
protein localization to bicellular tight junction
positive regulation of intracellular signal transduction
positive regulation of establishment of endothelial barrier
regulation of establishment of cell polarity
negative regulation of endothelial cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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