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Invitrogen
GABRB3 Monoclonal Antibody (N87/25), FITC
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FIGURE: 1 / 3
GABRB3 Antibody (MA5-45466) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with GABA-A Receptor Beta 3 monoclonal antibody (Product # MA5-45466) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor ... View More
Product Details
MA5-45466
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N87/25
Immunogen
Fusion protein amino acids 370-433 of mouse GABA-A-R-Beta 3
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45466 was sufficient for detection of Beta 3 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 55kDa. No cross-reactivity against GABA-A-R-Beta 2 or -Beta1.
Target Information
Gamma-amino butyric acid (GAMA) is a strong inhibitor of neuron transmission and is found throughout the central nervous system. GABA is formed by the decarboxylation of glutamate, resulting in the formation of one of two distinct types; GABA-A or GABA-B.The binding of GABA to GABA-A receptors results in the increase transportation of chlorine ions into the neuron. This influx results in signal transmission repression. Studies have shown that if cell to cell signaling goes unchecked due to a lack of GABA, a system overload can resulting in blackouts, rapid body contractions, seizures and epilepsy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cleft palate 1; GABA(A) receptor subunit beta-3; GABA-alpha receptor beta-2 subunit; GABAA receptor beta-3 subunit; GABAAR subunit beta-3; GABR B3; gamma-aminobutyric acid; gamma-aminobutyric acid (GABA) A receptor, beta 3; gamma-aminobutyric acid (GABA-A) receptor, subunit beta 3; gamma-aminobutyric acid A receptor beta 3; Gamma-aminobutyric acid receptor beta 3; Gamma-aminobutyric acid receptor subunit beta-3; gamma-aminobutyric acid receptor, subunit beta 3; gamma-aminobutyric acid type A receptor beta3 subunit; gamma-aminobutyric acid type A receptor subunit beta 3; MGC9051; testis gamma-aminobutyric acid receptor subunit beta 3; unnamed protein product
Gene Aliases: A230092K12Rik; AW049585; beta3; Cp1; DEE43; ECA5; EIEE43; Gabrb-3; GABRB3
UniProt ID: (Human) P28472, (Mouse) P63080, (Rat) P63079
Entrez Gene ID: (Human) 2562, (Mouse) 14402, (Rat) 24922
transmembrane signaling receptor activity
GABA-A receptor activity
ion channel activity
extracellular ligand-gated ion channel activity
chloride channel activity
GABA-gated chloride ion channel activity
identical protein binding
transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential
AP-2 adaptor complex binding
protein binding
ligand-gated ion channel
ion transport
chloride transport
signal transduction
gamma-aminobutyric acid signaling pathway
chemical synaptic transmission
nervous system development
brain development
learning
memory
response to xenobiotic stimulus
reproductive behavior
cerebellum development
ion transmembrane transport
social behavior
exploration behavior
circadian sleep/wake cycle, REM sleep
neuron development
synaptic transmission, GABAergic
palate development
hard palate development
inhibitory postsynaptic potential
inner ear receptor cell development
innervation
motor behavior
cellular response to zinc ion
cellular response to histamine
response to anesthetic
cochlea development
chloride transmembrane transport
inhibitory synapse assembly
transport
sensory perception of sound
regulation of neuron apoptotic process
negative regulation of neuron apoptotic process
negative regulation of neuron death
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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