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Invitrogen
ATP7A Monoclonal Antibody (L60/4), PE
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FIGURE: 1 / 3
ATP7A Antibody (MA5-45521) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using NIH 3T3 (NIH 3T3). Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Copper Transporting ATPase 1 monoclonal antibody (Product # MA5-45521) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum, Cytoplasm, Golgi Apparatus, Trans-Golgi Network Membrane, ... View More
Product Details
MA5-45521
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
L60/4
Immunogen
Synthetic peptide amino acids 42-61 (cytoplasmic C-terminus) of human Copper- transporting ATPase1
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45521 was sufficient for detection of Copper-transporting ATPase1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat IgG:HRP as the secondary antibody.|Detects approximately 180kDa in rat brain membrane preparations.
This antibody was formerly sold as clone S60-4.
Target Information
ATP7A (also known as Copper-transporting ATPase 1) functions as a transmembrane copper-translocating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Polarized epithelial cells such as Madin-Darby canine kidney cells are a physiologically relevant model for systemic copper absorption and reabsorption in vivo. Although ATP7A is not detectable in most normal tissues, it is expressed in a considerable fraction of many common tumor types. Increased expression of ATP7A renders cells resistant to cisplatin and carboplatin. Mutations in the ATP7A gene result in Menkes disease, which is fatal in early childhood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP 7A; ATP7A; ATPase, Cu++ transporting, alpha polypeptide; Copper pump 1; Copper-transporting ATPase 1; Cu++-transporting P-type ATPase; FLJ17790; MC 1; Menke; Menkes disease-associated protein; Menkes disease-associated protein homolog; Menkes protein; Menkes syndrome; OHS; OTTHUMP00000062077; unnamed protein product
Gene Aliases: ATP7A; DSMAX; HMNX; MC1; MK; MNK; SMAX3
UniProt ID: (Human) Q04656, (Mouse) Q64430, (Rat) P70705
Entrez Gene ID: (Human) 538, (Mouse) 11977, (Rat) 24941
nucleotide binding
copper ion transmembrane transporter activity
copper ion binding
protein binding
ATP binding
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism
superoxide dismutase copper chaperone activity
ATPase activity
cation-transporting ATPase activity
copper-dependent protein binding
copper-transporting ATPase activity
metal ion binding
P-type monovalent copper transporter activity
cuprous ion binding
copper-exporting ATPase activity
hydrolase activity
molecular_function
primary active transporter
blood vessel development
release of cytochrome c from mitochondria
blood vessel remodeling
regulation of oxidative phosphorylation
tryptophan metabolic process
tyrosine metabolic process
catecholamine metabolic process
ion transport
cation transport
copper ion transport
cellular copper ion homeostasis
mitochondrion organization
locomotory behavior
glycoprotein biosynthetic process
detoxification of copper ion
regulation of gene expression
copper ion import
removal of superoxide radicals
cerebellar Purkinje cell differentiation
pyramidal neuron development
central nervous system neuron development
extracellular matrix organization
collagen fibril organization
hair follicle morphogenesis
T-helper cell differentiation
epinephrine metabolic process
norepinephrine metabolic process
dopamine metabolic process
norepinephrine biosynthetic process
serotonin metabolic process
pigmentation
negative regulation of neuron apoptotic process
skin development
negative regulation of catecholamine metabolic process
ATP metabolic process
positive regulation of melanin biosynthetic process
elastic fiber assembly
lung alveolus development
neuron projection morphogenesis
dendrite morphogenesis
cartilage development
neuron apoptotic process
establishment of localization in cell
copper ion export
neuron cellular homeostasis
transport
plasma membrane copper ion transport
peptidyl-lysine modification
metal ion transport
hindlimb morphogenesis
positive regulation of catalytic activity
negative regulation of catalytic activity
response to copper ion
positive regulation of oxidoreductase activity
elastin biosynthetic process
in utero embryonic development
lactation
response to iron(III) ion
response to zinc ion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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