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Invitrogen
GABRA2 Monoclonal Antibody (N399/19), PE
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FIGURE: 1 / 4
GABRA2 Antibody (MA5-45728) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with GABA-A Receptor Alpha2 monoclonal antibody (Product # MA5-45728) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000; 1:5,000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin... View More
Product Details
MA5-45728
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N399/19
Immunogen
Fusion protein amino acids 350-385 (Cytoplasmic C-terminus) of rat GABA-A Receptor Alpha2
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:100 dilution of MA5-45728 was sufficient for detection of GABA-A R, Alpha2 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |Detects approximately 55kDa. Does not cross-react with GABA-A Receptor Alpha1.
This antibody was formerly sold as clone S399-19.
Target Information
GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ97076; GABA(A) receptor; GABA(A) receptor subunit alpha-2; GABAAR subunit alpha-2; GABAARalpha2; gamma-aminobutyric acid; gamma-aminobutyric acid (GABA) A receptor alpha 2 isoform GABRA2_1; gamma-aminobutyric acid (GABA) A receptor alpha 2 isoform GABRA2_2; gamma-aminobutyric acid (GABA) A receptor alpha 2 isoform GABRA2_3; gamma-aminobutyric acid (GABA) A receptor alpha 2 isoform GABRA2_4; gamma-aminobutyric acid (GABA) A receptor alpha 2 isoform GABRA2_5; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 2; gamma-aminobutyric acid receptor A2 subunit; Gamma-aminobutyric acid receptor subunit alpha-2; gamma-aminobutyric acid type A receptor alpha2 subunit; unnamed protein product
Gene Aliases: C630048P16Rik; DEE78; EIEE78; Gabra-2; GABRA2
UniProt ID: (Human) P47869, (Mouse) P26048
Entrez Gene ID: (Human) 2555, (Mouse) 14395, (Rat) 289606
transmembrane signaling receptor activity
GABA-A receptor activity
ion channel activity
extracellular ligand-gated ion channel activity
chloride channel activity
protein binding
benzodiazepine receptor activity
GABA-gated chloride ion channel activity
signaling receptor activity
ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential
transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential
ligand-gated ion channel
ion transport
chloride transport
gamma-aminobutyric acid signaling pathway
ion transmembrane transport
synaptic transmission, GABAergic
regulation of postsynaptic membrane potential
regulation of presynaptic membrane potential
chloride transmembrane transport
inhibitory synapse assembly
regulation of neurotransmitter levels
transport
neurotransmitter transport
chemical synaptic transmission
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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