Description: This HMER5DS antibody recognizes human MerTK, known also as Mer or c-mer, a 170-210 kDa member of the TAM family of tyrosine kinase receptors that also includes Axl and Tyro3. MerTK is expressed on a subset of anti-inflammatory macrophages and is involved in the removal of apoptotic cells. This process relies on two soluble ligands of Mer,TK Protein S and Gas6, that coat the surface of cells undergoing apoptosis. Upon binding these ligands, MerTK undergoes autophosphorylation at multiple tyrosine residues that activate the PI3K and Akt pathways. This results in the phagocytosis of target cells and also the direct inhibition of TLR-induced production of pro-inflammatory cytokines by the phagocytes. Mer may also function as a putative entry receptor for filoviruses. Deficiency of MerTK causes general autoimmunity, inflammation, and accumulation of apoptotic bodies. MerTK can be released from the cell surface by metalloproteinases, and this process is significantly enhanced when macrophages are stimulated, e.g. with LPS. MerTK is often expressed on malignant cells, and may be implicated in immune evasion.
Applications Reported: This HMER5DS antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HMER5DS antibody has been pre-titrated and tested by flow cytometric analysis of human monocyte-derived macrophages. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: c-mer; c-mer proto-oncogene tyrosine kinase; MER; MER receptor tyrosine kinase; MGC133349; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK; RP38; STK kinase; Tyrosine-protein kinase Mer
Gene Aliases: c-Eyk; c-mer; MER; MERTK; RP38; Tyro12
UniProt ID: (Human) Q12866
Entrez Gene ID: (Human) 10461