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Invitrogen
RUNX1 Recombinant Rat Monoclonal Antibody (RXDMC), Alexa Fluor™ Plus 488
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FIGURE: 1 / 3
RUNX1 Antibody (740114TP488) in Flow
Flow cytometry analysis of RUNX1 Recombinant Rat Monoclonal Antibody (RXDMC), Alexa Fluor™ Plus 488 (Product # 740114TP488) was performed using Jurkat (log phase) and IMR-32 cells. Cells were fixed with eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00), permeabilized and stained with 1 µg of the RUNX1 Recombinant Rat Monoclonal Antibody (RXDMC) Alexa Fluor™ Plus488 (Product # 740114TP488 ). Higher expression of RUNX1 was observed in Jurkat (log phase) (pink histogram) in comparison to IMR-32 (blue histogram... View More
Product Details
740114TP488
Product Specifications
Species Reactivity
Human
Host/Isotype
Rat
/ IgG2a, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
RXDMC
Conjugate
Excitation/Emission Max
493/518 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
RUNX1 belongs to the mammalian RUNX family of DNA binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX1 is essential for hematopoietic stem cell generation in the vascular regions of the aorta, vitelline and umbilical arteries, yolk sac and placenta. RUNX1 is expressed in all tissues except brain and heart, with highest levels in thymus, bone marrow and peripheral blood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acute myeloid leukemia 1 protein; AML-1; aml1 oncogene; AML1-EVI-1 fusion protein; CBF-alpha 2; CBF-alpha-2; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; Oncogene AML-1; OTTHUMP00000108697; OTTHUMP00000108700; OTTHUMP00000108702; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; RUN1; Runt-related transcription factor 1; RUNX1/ZNF687 fusion; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; transcription factor
Gene Aliases: AML1; AML1-EVI-1; AMLCR1; CBF2alpha; CBFA2; EVI-1; PEBP2aB; PEBP2alpha; RUNX1
UniProt ID: (Human) Q01196
Entrez Gene ID: (Human) 861
regulatory region DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
core promoter binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
protein binding
ATP binding
DNA-binding transcription factor
immunoglobulin fold transcription factor
Runt transcription factor
gene-specific transcriptional regulator
ossification
chondrocyte differentiation
transcription from RNA polymerase II promoter
hemopoiesis
positive regulation of granulocyte differentiation
positive regulation of interleukin-2 production
positive regulation of angiogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
peripheral nervous system neuron development
hematopoietic stem cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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