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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:1,000 | - |
Immunocytochemistry (ICC/IF) |
1:50-1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide corresponding to amino acids 339-384 of human SCK |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.05% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2553883 |
This antibody detects endogenous protein at a molecular weight of 68 kDa.
Purity is >95% by SDS-PAGE.
Src homology 2 (SH2) domains bind specifically to tyrosine-phosphorylated proteins that temporally participate in signal transduction events. Shc-like protein (Sck) is a neuronal adaptor protein that contains an N-terminal PTB (phosphotyrosine binding) domain, a collagen homology (CH) domain, and a conserved C-terminal SH2 domain. Human Sck transcripts are present at high levels in liver, pancreas, prostate and ovary. In vascular endothelial cells, Sck participates in VEGF-induced signal transduction. Treatment of human umbilical vein endothelial (HUVEC) cells with VEGF induces recruitment of Sck to tyrosine-1175 of the kinase insert domain-containing receptor (KDR) and enhances Sck tyrosine phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: neuronal Shc adaptor homolog; Protein Sck; Protein Sli; SH2 domain protein C2; SHC (Src homology 2 domain containing) transforming protein 2; SHC-transforming protein 2; SHC-transforming protein B; src homology 2 domain-containing transforming protein C2; Src homology 2 domain-containing-transforming protein C2
Gene Aliases: 6720466E06; SCK; SHC2; SHCB; SLI
UniProt ID: (Human) P98077, (Mouse) Q8BMC3, (Rat) O70142
Entrez Gene ID: (Human) 25759, (Mouse) 216148, (Rat) 314612
Molecular Function:
scaffold/adaptor protein
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