Immunofluorescent analysis of SETDB2 showing staining in the nucleus of HCT116 cells. HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a SETDB2 polyclonal antibody (Product # PA5-30848) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 1 and 160 of Human SETDB2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30848 targets SETDB2 in WB applications and shows reactivity with Human samples.
The PA5-30848 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 160 of Human SETDB2.
Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 (see MIM 601128) methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity (Zhang et al., 2003 [PubMed 12754510]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.