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Agrisera
XLG2/Extra-large G-protein 2 Polyclonal Antibody
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FIGURE: 1 / 1
XLG2/Extra-large G-protein 2 Antibody (AS22-4881) in WB
Samples: Left panel: 50 ng of recombinant At XLG 2 protein Right panel: 20 µg of a total protein from Arabidopsis thaliana wt (wt) and xlg 1,2,3 goa 1-3 quadruple mutant (mut)Samples were separated on 12% SDS-PAGE and blotted for 1 h to PVDF using wet transfer. Blot was blocked with 5% milk for 1 h/RT. Blot was incubated in the primary antibody at a dilution of 1:5,000 for 1 h/4°C with agitation in TBS-T. T... View More
Product Details
AS22-4881
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant XLG2 of Arabidopsis thaliana.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
XLG2 encodes a plant-specific, noncanonical heterotrimeric G protein alpha-like factor that localizes dynamically to the nucleus and cell periphery, consistent with roles in both intracellular signaling and transcriptional control. The protein is structurally distinguished from canonical G alpha subunits by a large N-terminal extension (approximately 400 amino acids) that contains regulatory features including a functional nuclear localization signal, coupled to a C-terminal G alpha-like domain capable of nucleotide binding and hydrolysis. Biochemically, XLG2 binds and hydrolyzes GTP in a Ca2+-dependent manner (rather than the typical Mg2+ dependence of many metazoan G alpha proteins), supporting its classification as an unconventional GTPase. Functionally, XLG2 participates in defense signaling: it is induced during bacterial infection, contributes to pathogen resistance, and can act within an alternative G protein complex via interaction with the Arabidopsis G beta subunit, shaping downstream immune gene activation. In development, XLG2 can interact with the nuclear factor RTV1 and enhance its DNA-binding activity, thereby promoting transcription of floral integrator genes such as FT and SOC1 and influencing flowering time.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT4G34390; extra-large GTP-binding protein 2; F10M10.160; F10M10_160
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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