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Immunofluorescence analysis of ZO-1 / TJP1 Antibody, FITC conjugate (ZO1-1A12) was done on 90% confluent log phase CaCo2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ZO-1 / TJP1 Antibody, FITC conjugate (ZO1-1A12)(339111) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cell junctional localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Dog, Human|
|Published species reactivity||Dog, Rat, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant ZO-1 fusion protein encompassing amino acids 334-634|
|Storage buffer||PBS, pH 7.4, with 50% glycerol, 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane- associated guanylate kinase (MAGUK) family and include occludin, ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
EB1 regulates tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier in male rats: an in vitro study.
33-9111 was used in immunohistochemistry - frozen section to study the role of EB1 in tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier.
|Tang EI,Mok KW,Lee WM,Cheng CY||Endocrinology (156:680)||2015|
|Human||Not Cited||Hailey-Hailey disease and tight junctions: Claudins 1 and 4 are regulated by ATP2C1 gene encoding Ca(2+) /Mn(2+) ATPase SPCA1 in cultured keratinocytes.||Raiko L,Siljamäki E,Mahoney MG,Putaala H,Suominen E,Peltonen J,Peltonen S||Experimental dermatology (21:586)||2012|
Protective effects of nonionic triblock copolymers on bile acid-mediated epithelial barrier disruption.
33-9111 was used in immunocytochemistry to examine the ability of nonionic block copolymers to abrogate barrier failure in response to bile acid exposure.
|Edelstein A,Fink D,Musch M,Valuckaite V,Zaborina O,Grubjesic S,Firestone MA,Matthews JB,Alverdy JC||Shock (Augusta, Ga.) (36:451)||2011|
|Dog||1:100||Apical localization of a functional TRPC3/TRPC6-Ca2+-signaling complex in polarized epithelial cells. Role in apical Ca2+ influx.||Bandyopadhyay BC,Swaim WD,Liu X,Redman RS,Patterson RL,Ambudkar IS||The Journal of biological chemistry (280:12908)||2005|
Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1
TJP1; ZO-1; ZO1