Immunofluorescence analysis of ZO-1 / TJP1 Antibody, FITC conjugate (ZO1-1A12) was done on 90% confluent log phase CaCo2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ZO-1 / TJP1 Antibody, FITC conjugate (ZO1-1A12)(339111) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cell junctional localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Dog, Human|
|Published species reactivity||Dog, Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human recombinant ZO-1 fusion protein encompassing amino acids 334-634|
|Storage buffer||PBS, pH 7.4, with 50% glycerol, 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 6 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 4 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Western Blot (WB)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane- associated guanylate kinase (MAGUK) family and include occludin, ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The retinal phenotype of Grk1-/- is compromised by a Crb1 rd8 mutation.
33-9111 was used in immunohistochemistry to characterize Grk1 knock out mice with or without the Crb1(rd8) mutation
|Pak JS,Lee EJ,Craft CM||Molecular vision (21:1281)||2015|
A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium.
33-9111 was used in immunocytochemistry to use a multiplex high-throughput gene expression assay to detect endogenous expression of multiple developmental, functional, and disease markers in iPS cell-derived retinal pigment epithelium.
|Ferrer M,Corneo B,Davis J,Wan Q,Miyagishima KJ,King R,Maminishkis A,Marugan J,Sharma R,Shure M,Temple S,Miller S,Bharti K||Stem cells translational medicine (3:911)||2014|
A novel human endogenous retroviral protein inhibits cell-cell fusion.
33-9111 was used in immunocytochemistry to identify the first host cell-encoded protein, suppressyn, that inhibits cell fusion in mammals.
|Sugimoto J,Sugimoto M,Bernstein H,Jinno Y,Schust D||Scientific reports (3:null)||2013|
Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF.
33-9111 was used in immunocytochemistry to study the immune responses of uncomplicated versus cerebral malaria patients
|Wassmer SC,Moxon CA,Taylor T,Grau GE,Molyneux ME,Craig AG||Cellular microbiology (13:198)||2011|
Adjudin-mediated Sertoli-germ cell junction disassembly affects Sertoli cell barrier function in vitro and in vivo.
33-9111 was used in immunocytochemistry to test if the function of the blood-testis barrier in affected by adjudin
|Su L,Cheng CY,Mruk DD||The international journal of biochemistry and cell biology (42:1864)||2010|
Adhering junction dynamics in the testis are regulated by an interplay of beta 1-integrin and focal adhesion complex-associated proteins.
33-9111 was used in immunohistochemistry to study the regulation of adheren junction assembly.
|Siu MK,Mruk DD,Lee WM,Cheng CY||Endocrinology (144:2141)||2003|
EB1 regulates tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier in male rats: an in vitro study.
33-9111 was used in immunohistochemistry - frozen section to study the role of EB1 in tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier.
|Tang EI,Mok KW,Lee WM,Cheng CY||Endocrinology (156:680)||2015|
|Not Applicable||Not Cited||
Mesoderm is required for coordinated cell movements within zebrafish neural plate in vivo.
33-9111 was used in immunohistochemistry - frozen section to analyze how cell movements in zebrafish neural plate require mesoderm in vivo
|Araya C,Tawk M,Girdler GC,Costa M,Carmona-Fontaine C,Clarke JD||Neural development (9:null)||2014|
Effects of di(2-ethylhexyl) phthalate on gap and tight junction protein expression in the testis of prepubertal rats.
33-9111 was used in immunohistochemistry - frozen section and western blot to test if di(2-ethylhexyl) phthalate alters the expression of testicular gap and tight junction proteins
|Sobarzo CM,Lustig L,Ponzio R,Suescun MO,Denduchis B||Microscopy research and technique (72:868)||2009|
Disruption of Sertoli-germ cell adhesion function in the seminiferous epithelium of the rat testis can be limited to adherens junctions without affecting the blood-testis barrier integrity: an in vivo study using an androgen suppression model.
33-9111 was used in immunohistochemistry - frozen section to study regulation of the blood-testis barrier
|Xia W,Wong CH,Lee NP,Lee WM,Cheng CY||Journal of cellular physiology (205:141)||2005|
Rai14 (retinoic acid induced protein 14) is involved in regulating f-actin dynamics at the ectoplasmic specialization in the rat testis*.
33-9111 was used in immunohistochemistry to report that Rai14 regulates actin filaments organization in Sertoli cells during the epithelial cycle
|Qian X,Mruk DD,Cheng CY||PloS one (8:null)||2013|
|Not Applicable||Not Cited||
Localization of semaphorin 3A in the rat cornea.
33-9111 was used in immunohistochemistry to study the localization of Sema3A in the cornea
|Morishige N,Ko JA,Liu Y,Chikama T,Nishida T||Experimental eye research (86:669)||2008|
Tumor necrosis factor ¿-mediated restructuring of the Sertoli cell barrier in vitro involves matrix metalloprotease 9 (MMP9), membrane-bound intercellular adhesion molecule-1 (ICAM-1) and the actin cytoskeleton.
33-9111 was used in immunocytochemistry to examine the effects of TNFalpha on Sertoli cell barrier function
|Lydka M,Bilinska B,Cheng CY,Mruk DD||Spermatogenesis (2:294)||2012|
Protective effects of nonionic triblock copolymers on bile acid-mediated epithelial barrier disruption.
33-9111 was used in immunocytochemistry to examine the ability of nonionic block copolymers to abrogate barrier failure in response to bile acid exposure.
|Edelstein A,Fink D,Musch M,Valuckaite V,Zaborina O,Grubjesic S,Firestone MA,Matthews JB,Alverdy JC||Shock (Augusta, Ga.) (36:451)||2011|
|Not Applicable||Not Cited||
Effect of low fluence diode laser irradiation on the hydraulic conductivity of perfused trabecular meshwork endothelial cell monolayers.
33-9111 was used in immunocytochemistry to examine the effect of low-fluence diode laser irradiation upon the fluid perfusion characteristics of cultured human trabecular meshwork cell monolayers
|Roberts CJ,Rivera BK,Grzybowski DM,Mahmoud AM,Weber PA||Current eye research (32:625)||2007|
|Human||Not Cited||Hailey-Hailey disease and tight junctions: Claudins 1 and 4 are regulated by ATP2C1 gene encoding Ca(2+) /Mn(2+) ATPase SPCA1 in cultured keratinocytes.||Raiko L,Siljamäki E,Mahoney MG,Putaala H,Suominen E,Peltonen J,Peltonen S||Experimental dermatology (21:586)||2012|
A mouse model for interstitial cystitis/painful bladder syndrome based on APF inhibition of bladder epithelial repair: a pilot study.
33-9111 was used in immunohistochemistry - paraffin section to assess the ability of a synthetic glycopeptide antiproliferative factor derivative to inhibit bladder epithelial repair in mice
|Keay S,Leitzell S,Ochrzcin A,Clements G,Zhan M,Johnson D||BMC urology (12:null)||2012|
|Not Applicable||Not Cited||
Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
33-9111 was used in flow cytometry to isolate and characterize multipotent mesenchymal stem cells from term human placenta
|Semenov OV,Koestenbauer S,Riegel M,Zech N,Zimmermann R,Zisch AH,Malek A||American journal of obstetrics and gynecology (202:193.e1)||2010|
|Dog||1:100||Apical localization of a functional TRPC3/TRPC6-Ca2+-signaling complex in polarized epithelial cells. Role in apical Ca2+ influx.||Bandyopadhyay BC,Swaim WD,Liu X,Redman RS,Patterson RL,Ambudkar IS||The Journal of biological chemistry (280:12908)||2005|