General Purpose HPLC Columns for Protein Analysis

Thermo Scientific POROS bead technology revolutionizes biomolecule purification and analysis with its robust polymer matrix, offering exceptional mechanical stability and high flow rates for HPLC applications. The unique structure of POROS beads creates a high surface area, facilitating exceptional binding capacity and rapid mass transfer, ultimately contributing to faster processing times and higher throughput. Its versatility supports a wide range of ligands, making it an indispensable and powerful technology for applications in biotechnology, pharmaceuticals, and life sciences.

Ion exchange chromatography is a method used to separate ionic molecules such as proteins based on their charge. In this technique, a sample containing the molecule of interest is injected into the chromatography system and passed through a column filled with a resin that has charged functional groups. These groups interact with the charged molecules in the sample. A liquid mobile phase is used to carry the sample through the column, and the ions are separated based on their affinity to the resin. A counter ion such as sodium or chloride is used to displace or exchange the molecule bound to the surface of the resin. The concentration of counter ion needed depends on how strongly the molecule binds to the resin. As the molecules elute from the column, they are detected and quantified.