miRNA Analysis Using TaqMan Assays
Which TaqMan miRNA Assay is right for you?
Choosing the best TaqMan miRNA Assay product to suit your needs depends on different factors, including the instrument available to you and the number of samples and targets being interrogated. Consider the convenience of predesigned and pre-plated assays to save time in the lab.
There are now even more choices and formats for detection of mature, biologically active miRNA, designed to support a growing range of miRNA applications. TaqMan miRNA Assays have been configured using long-standing bioinformatics expertise in ultra-specific primer and probe design. If you are studying a novel target or a non-model organism then you can use the Custom TaqMan Assay Design Tool to design and order a Custom TaqMan Gene Expression Assay to detect any gene from any organism.
TaqMan MicroRNA assays employ a novel target-specific stem–loop primer (miRNA-specific RT) during cDNA synthesis to produce a template for real-time PCR
TaqMan Advanced miRNA assays employ a universal RT step for a streamlined workflow, and a universal miR-Amp step to enable highly sensitive detection by real-time PCR
Choose from a variety of formats and configurations to meet all your different research needs. Whether you are looking at a single target or thousands of samples, there is a TaqMan Assay format for you.
- Low entry price
- Run on any real-time PCR instrument
384-well microfluidic cards
- Low cost per reaction
- Optimal for medium to large projects
- Run on Applied Biosystems™ QuantStudio™ 7 Flex & 12K Flex, ViiA™ 7, and 7900HT Fast real-time PCR systems
- Optimal for small to medium projects
- Balances flexibility with streamlined reaction setup
- Run on any 96-well real-time PCR instrument
- Lowest cost for large projects
- Ultimate throughput
- Run on QuantStudio 12K Flex real-time PCR system
If you are planning on duplexing or multiplexing, make sure that the reporter dyes you choose are compatible with your qPCR instrument model. Each instrument must have an excitation source to excite the fluorescent dye and a detector to detect the fluorescent emissions. The excitation and emission filters support different types of dyes, and the number of these filters can vary depending upon the qPCR instrument model chosen.
For miRNA studies, selection of a valid endogenous control is critical for normalization and corrects for inter-sample variation. We offer endogenous controls in all formats to help you determine the best control gene for your study.
TaqMan miRNA Assay selection tool
Find the right TaqMan miRNA Assay for your needs.
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For Research Use Only. Not for use in diagnostic procedures.