TaqMan Advanced miRNA Assays

Applied Biosystems TaqMan Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR. Together with the TaqMan Advanced miRNA cDNA Synthesis Kit, this solution is designed to provide a streamlined workflow that allows for exceptional detection of multiple miRNA targets from a single sample.

  • Universal reverse transcription (RT)—one RT step for all TaqMan Advanced miRNA Assays.
  • Sensitive— Detect targets with as few as 60 copies of input miRNA into the cDNA synthesis reaction.
  • Specific—detect only mature miRNA and distinguish related highly homologous miRNAs with gold-standard TaqMan probe specificity.
  • Small sample input—detect and quantify mature miRNA from as little as 1 pg of total RNA or 2 μL of purified plasma or serum.
  • Versatile—compatible with tissue and biofluids including serum and plasma.
  • Fast delivery—all pre-designed assays ship out in 1–2 business days


Already know your target of interest?

Search for a pre-designed TaqMan Advanced miRNA Assay using the tool below:

Looking to design your own assay? Click here to learn more about our offerings, and to request a design for a custom TaqMan Advanced miRNA Assay when a pre-designed assay is not offered.

Availability & sizes: Inventoried assays are pre-packaged in "Small" scale and are ready to ship.

taqman-array-cards-plate

Now available: TaqMan Advanced miRNA assays in card, plate, and OpenArray format


Taqman Advanced miRNA Product Selection Guide

Format

Recommended TaqMan assay/array

Individual qPCR Assays

New TaqMan Advanced miRNA Assays
Comprehensive collection of pre-designed TaqMan qPCR assays for human, mouse and rat species

All pre-designed assays are inventoried and ship out in 1-2 days

Search for pre-designed assays ›

96-well plate

TaqMan Advanced miRNA  96 well array plates
Comprehensive collection of pre-plated, pre-designed panels available in both fast and standard 96 well formats.

Search for pre-designed 96 well plates ›

384-well microfluidic TaqMan Array card

TaqMan Advanced miRNA array cards
Small reaction volumes and streamlined workflow enable profiling up to 380 miRNA targets from as little as 1 ng of total RNA

Search for predesigned 384-well arrays ›
Design your own custom 384-well array ›

OpenArray plateTaqMan Advanced miRNA OpenArray panels
Run three samples per QuantStudio 12K Flex OpenArray plate, translating up to 818 assays per sample (up to 758 unique assays)

Taqman™ OpenArray Human Advanced MicroRNA Panel
Contact: QuantStudioFrontDesk@lifetech.com
Custom 384 well platesContact: specialty_plates@thermofisher.com

Workflow

mirVana™ miRNA isolation kits

mirVana miRNA Isolation Kits

mirVana miRNA Isolation kits use a rapid procedure to isolate total RNA, including small RNAs such as miRNA, small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells.

TaqMan Advanced miRNA cDNA Synthesis kit

TaqMan Advanced miRNA cDNA Synthesis Kit

The TaqMan Advanced miRNA cDNA Synthesis Kit is used for preparation of cDNA templates from the entire pool of mature miRNAs in a sample.

TaqMan Advanced miRNA cDNA Synthesis Kit

miR-Amp Universal Amplification

miR-Amp uses universal primers and master mix to uniformly increase the amount of cDNA for each target. miR-Amp reagents are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.

Applied Biosystems real-time PCR instruments

Applied Biosystems real-time PCR instruments

With Applied Biosystems real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments.

mirVana™ miRNA isolation kits

mirVana miRNA Isolation Kits

mirVana miRNA Isolation kits use a rapid procedure to isolate total RNA, including small RNAs such as miRNA, small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells.

TaqMan Advanced miRNA cDNA Synthesis kit

TaqMan Advanced miRNA cDNA Synthesis Kit

The TaqMan Advanced miRNA cDNA Synthesis Kit is used for preparation of cDNA templates from the entire pool of mature miRNAs in a sample.

TaqMan Advanced miRNA cDNA Synthesis Kit

miR-Amp Universal Amplification

miR-Amp uses universal primers and master mix to uniformly increase the amount of cDNA for each target. miR-Amp reagents are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.

Applied Biosystems real-time PCR instruments

Applied Biosystems real-time PCR instruments

With Applied Biosystems real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments.


Additional information

SpeciesTaqMan Advanced miRNA assays are available for human, mouse, and rat miRNAs. We expect to increase the number of miRNA assays in alignment with the Sanger miRBase Registry.
Assay Components

TaqMan Advanced miRNA assays are delivered in a single tube containing the specific pre-formulated TaqMan Assay (TaqMan MGB probe, and forward and reverse primers).

Note: TaqMan Advanced miRNA cDNA Synthesis Kit (Cat. No. A28007)  and TaqManFast Advanced Master Mix (Cat. No. 4444556) are recommended for use with TaqMan Advanced miRNA assays.

Available Sizes
ProductCatalog no.Number of reactions
TaqMan Advanced miRNA assaysA25576250 qPCR reactions (20 μL)
Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to—25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

SpeciesTaqMan Advanced miRNA assays are available for human, mouse, and rat miRNAs. We expect to increase the number of miRNA assays in alignment with the Sanger miRBase Registry.
Assay Components

TaqMan Advanced miRNA assays are delivered in a single tube containing the specific pre-formulated TaqMan Assay (TaqMan MGB probe, and forward and reverse primers).

Note: TaqMan Advanced miRNA cDNA Synthesis Kit (Cat. No. A28007)  and TaqManFast Advanced Master Mix (Cat. No. 4444556) are recommended for use with TaqMan Advanced miRNA assays.

Available Sizes
ProductCatalog no.Number of reactions
TaqMan Advanced miRNA assaysA25576250 qPCR reactions (20 μL)
Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to—25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

For Research Use Only. Not for use in diagnostic procedures.