TaqMan Advanced miRNA Assays

Applied Biosystems TaqMan™ Advanced miRNA Assays enable highly sensitive and specific quantification of mature miRNAs using qPCR. Together with the Applied Biosystems TaqMan Advanced miRNA cDNA Synthesis Kit, this solution provides a streamlined upfront workflow that is ideal for analysis of multiple miRNA targets from a single sample or low-level RNA samples such as serum and plasma. 

  • Universal RT—one RT step for all TaqMan Advanced miRNA Assays
  • Sensitivity—detect as few as 60 copies input into cDNA synthesis
  • Specificity—detect only the mature miRNA and distinguish between highly homologous targets
  • Small sample input—detect and quantify mature miRNA from as little as 1pg of total RNA or 2 µL plasma
  • Compatibility with biofluids including human serum, plasma, and tissue

TaqMan Advanced miRNA Assays on cards and plates

Be one of the first to use our TaqMan Advanced miRNA chemistry on a TaqMan Array Card or OpenArray Plate. Enable highly sensitive and specific quantification of miRNAs in a high throughput format.
Request early access

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Figure 1: Dynamic range and sensitivity.

Superior sensitivity

The TaqMan Advanced miRNA system effectively manages the typically low RNA amounts of serum and plasma starting materials. Unlike traditional pre-amplification, the miR-Amp primers in the ligation-based cDNA synthesis kit recognize the universal sequences added to every miRNA on the 5’ and 3’ ends, to help prevent amplification bias. Representing some of the most sensitive and specific assays available, TaqMan Advanced miRNA Assays accurately detect as few as 60 copies from as little as 1pg of total RNA or 2 µL of serum/plasma.

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Figure 2: Assay specificity using closely
related miRNAs.

Gold-standard TaqMan specificity

TaqMan Advanced miRNA Assays easily discriminate between closely related mature miRNAs differing by as little as one base. The TaqMan Advanced miRNA system goes even further to offer superior levels of specificity and sensitivity with closely related miRNAs that differ by only 1 bp at the 5’end—the highly conserved "seed region" of homologous miRNAs. 

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Universal reverse transcription

The TaqMan Advanced miRNA cDNA Synthesis Kit has a universal reverse transcription (RT) step to simplify the upfront workflow. Using 3’ poly-A tailing and 5’ ligation of an adaptor sequence to extend the miRNA on each end prior to reverse transcription, the cDNA is universally amplified using miR-Amp primers and miR-Amp master mix to uniformly increase the amount of cDNA.

The kit is ideal for samples that:

  • Have multiple miRNA targets from a single amplified sample
  • Are limited in quantity (e.g., serum, plasma, or other biological fluids)
Workflow

mirVana miRNA Isolation Kits

Invitrogen™ mirVana™ miRNA Isolation Kits use a rapid procedure to isolate total RNA, including small RNAs such as miRNA, small interfering RNA (siRNA), and small nuclear RNA (snRNA), from tissues and cells.

TaqMan Advanced miRNA cDNA Synthesis Kit

The TaqMan Advanced miRNA cDNA Synthesis Kit is used for preparation of cDNA templates from the entire pool of mature miRNAs in a sample.

miR-Amp Universal Amplification

miR-Amp uses universal primers and master mix to uniformly increase the amount of cDNA for each target. miR-Amp reagents are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.

Applied Biosystems real-time PCR instruments

With Applied Biosystems™ real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems™ TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments.

   

Species TaqMan Advanced miRNA Assays are available for human miRNAs. We expect to increase the number of miRNA assays in alignment with the Sanger miRBase Registry.
Assay Components

TaqMan Advanced miRNA Assays are delivered in a single tube containing the specific pre-formulated TaqMan Assay (TaqMan probe and PCR primer set).

Available Sizes
Product Catalog no. Number of reactions
TaqMan Advanced miRNA Assays A25576 250 qPCR reactions (20 μL)
Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to –25ºC.

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