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Figure 1: Dynamic range and sensitivity.
The TaqMan Advanced miRNA system effectively manages the typically low RNA amounts of serum and plasma starting materials. Unlike traditional pre-amplification, the miR-Amp primers in the ligation-based cDNA synthesis kit recognize the universal sequences added to every miRNA on the 5’ and 3’ ends, to help prevent amplification bias. Representing some of the most sensitive and specific assays available, TaqMan Advanced miRNA Assays accurately detect as few as 60 copies from as little as 1pg of total RNA or 2 µL of serum/plasma.