OpenArray™ plate technology on the QuantStudio™ 12K Flex Real-Time System helps streamline microRNA (miRNA) profiling, at a scale previously unattainable. Achieve the highest sample throughput for mid-density miRNA profiling—in a single work day, one user can process 48 samples with minimal pipetting steps and without the use of robotics.

  • Broad coverage: run 3 samples per QuantStudio 12K Flex OpenArray Plate translating up to 818 assays per sample (up to 758 unique assays)
  • High throughput: 12 samples per ~2 hr instrument run and 48 samples per 8hr working day
  • Cost effective: rapid sample screening with minimal hands-on time
  • Minimal sample requirement: only 200 ng of total RNA needed for a full profile

TaqMan Advanced miRNA Assays on cards and plates

Be one of the first to use our TaqMan Advanced miRNA chemistry on a TaqMan Array Card or OpenArray Plate. Enable highly sensitive and specific quantification of miRNAs in a high throughput format.
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TaqMan MicroRNA using the OpenArray Real-Time PCR System experimental workflow

Life Technologies offers a variety of kits for the isolation and analysis of miRNA and other small RNAs, enabling quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).

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mirVana™ miRNA Isolation Kit
Isolate total RNA that contains small RNAs such as miRNA, siRNA, and snRNA. These small RNAs are often lost during traditional RNA isolation methods.

Designed to streamline miRNA profiling, Megaplex™ RT Primers are pools of target-specific, stem-loop reverse transcription (RT) primers from TaqMan® MicroRNA Assays. These RT-primer pools are ideal for global miRNA expression profiling; just two pools provide comprehensive coverage of the Sanger miRBase v14 and v15 database content for human and rodent, respectively. The content of each pool matches corresponding QuantStudio™ 12K Flex TaqMan® OpenArray® Human or Rodent MicroRNA Panels. The TaqMan® MicroRNA RT Kit provides optimal TaqMan® MicroRNA Assay performance. This kit is used to convert miRNA to cDNA prior to real-time PCR quantitation.

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Megaplex™ RT Primers
Whether your profiling experiment requires ultimate sensitivity, broad coverage, or both, Megaplex™ Primer Pools provide the flexibility to reach your research goals. Megaplex™ Primer Pools provide comprehensive coverage of miRNAs in the Sanger miRBase database, and when used with TaqMan® MicroRNA Arrays, offer an ideal miRNA profiling solution.

 

TaqMan® MicroRNA Reverse Transcription Kit
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance of TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.

Megaplex™ PreAmp Primers help you stretch as little as 1 ng of input total RNA into enough cDNA to generate a comprehensive miRNA expression profile. With content matched to specific Megaplex™ RT Primers and QuantStudio™ 12K Flex TaqMan® OpenArray® Human MicroRNA Panels, cDNA prepared by reverse transcription can be preamplified using Megaplex™ PreAmp Primers and TaqMan® PreAmp Master Mix to amplify all miRNAs present in the original sample, significantly increasing the ability to detect low abundance transcripts.

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Megaplex™ PreAmp Primers
For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect miRNAs expressed at low levels, enabling the generation of a comprehensive expression profile using very little sample—as little as 1 ng of input total RNA.

 

TaqMan® PreAmp Master Mix
TaqMan® PreAmp Master Mix preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions. The TaqMan® PreAmp Master Mix Kit consists of TaqMan® PreAmp Master Mix and TaqMan® Gene Expression Master Mix.

Load sample mixes onto QuantStudio™ 12K Flex TaqMan® OpenArray® MicroRNA Panels using the QuantStudio™ 12K Flex OpenArray® AccuFill™ System. The plate bottom is encased with an alloy for easy handling. Simply fill with immersion fluid and apply the adhesive lid.

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TaqMan® OpenArray® MicroRNA Panels 
TaqMan® OpenArray® MicroRNA Panels contain preconfigured sets of TaqMan® MicroRNA Assays, for either human or rodent profiling studies, in the highest- throughput format available. With these panels, you can run 3 samples simultaneously, each with over 700 unique TaqMan® MicroRNA Assays.

 

TaqMan® OpenArray® Real-Time PCR Master Mix
TaqMan® OpenArray® Real-Time PCR Master Mix is conveniently formulated to support miRNA profiling using TaqMan® OpenArray® MicroRNA Panels on the QuantStudio™ 12K Flex Real-Time PCR System.

OpenArray® AccuFill™ System

The OpenArray® AccuFill™ System loads the sample mix from a 384‐well plate onto the TaqMan® OpenArray® Real-Time PCR plate with minimal human intervention.

OpenArray® Sample Tracker Software integrates the sample information from a 384‐well plate file with the assay information contained in the plate setup file. 

Perform a real-time plate read using the QuantStudio™ 12K Flex Real-Time PCR System.

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QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block
The QuantStudio™ 12K Flex Real-time PCR Systemand QuantStudio™ 12K Flex OpenArray® AccuFill™ System combination can process hundreds to thousands of samples per day, enabling mid-density, high-throughput results with ease of use and without robotics.

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RealTime StatMiner® Software
RealTime StatMiner® Software from Integromics is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. With just a single click, the software imports raw data or Ct values from any of our real-time PCR instruments, and then guides you though every step of analysis, giving you accurate results in minutes.

A recent study published in Nature found that the QuantStudio 12K Flex system with OpenArray plates outperforms other platforms for miRNA screening.

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