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Achieve higher yields and throughput, across a wider variety of proteins
Producing increasingly complex proteins with higher yields and increased throughput is critical to accelerating breakthroughs in protein research applications and drug development. To address these needs, we have developed the next-generation Gibco Expi293 Expression System.
The Gibco Expi293 PRO Expression System is a fully integrated expression system designed to deliver higher yields of a wider variety of proteins—including challenging and low-yield proteins—with exceptional speed, efficiency, and an easy-to-automate protocol.
Expi293 PRO Expression System components
The Expi293 PRO Expression System is a fully integrated and optimized HEK293 transient expression system. It includes high-density, high-performing HEK293 cells, chemically defined and animal-origin-free medium, and a complete transfection kit—all designed to deliver outstanding performance.
Expi293 PRO Expression System capabilities
Explore the capabilities of the Expi293 PRO Expression System by clicking on each icon to learn more.
High throughput and automation compatible
Simplified protocols and optimized reagents seamlessly integrate with small-scale and automated platforms.
Unlock new protein targets
The Expi293 PRO Expression System empowers you to express even the most difficult and traditionally low-yield proteins—including secreted, membrane, intracellular, and viral proteins. Push the boundaries of discovery and accelerate breakthroughs to shape the future of protein research and drug or vaccine development.
Figure 1. Expi293 PRO system increases expression of a diverse array of secreted non-antibodies by an average of 10x, with improvements up to 20x, versus Expi293 system.
Figure 2. Expi293 PRO system increases expression of secreted antibodies by an average of 4x versus Expi293 system.
Figure 3. Expi293 PRO system increases expression of membrane proteins by an average of 4x versus Expi293 system.
Figure 4. Expi293 PRO system increases expression of secreted viral proteins by an average of 6x, with improvements up to 11x, versus Expi293 system.
Figure 5. Expi293 PRO system increases expression of intracellular proteins by an average of 3.5x versus Expi293 system.
Figure 6. Expi293 PRO system increases expression of extracellular domain proteins by an average of 13x versus Expi293 system, and a canine IgG protein by 11x versus Expi293 system.
Exceptional protein yields from a transient 293 system
With the Expi293 PRO system, you can achieve protein yields of up to 5 g/L, setting a new standard for transient expression systems. This multi-fold improvement in productivity ensures you have the starting material you need, and quickly, to facilitate advanced studies and applications.
Figure 7. Expi293 PRO system increases expression across 40 monoclonal antibodies, with an average 4x improvement versus Expi293 system, and over 70% reaching >1 g/L.
Cost-effective solution
Optimize your budget and resources without compromising results. The Expi293 PRO system offers a cost-effective solution by enabling higher protein yields at the lowest cost per milligram, helping ensure that labs of all sizes have access to high-quality protein production. By reducing overall costs, the Expi293 PRO system helps enhance the value of your scientific investments.
Figure 8. Comparison of cost per milligram of Human IgG produced using three transfection workflows. Expi293 PRO (full system) achieves the lowest production cost at $0.83/mg*, making it over 2.5x more cost-effective than the standard Expi293 full system ($2.26/mg*) and over 4.5x more effective than using Expi293 PRO cells with a competitive transfection reagent ($3.71/mg*). Additional value beyond cost savings: reduced labor costs (less hands-on time for expression and purification) and sustainability (less plastic waste and reduced shipping materials per mg of protein). *For this comparison, prices are given in US Dollars (USD).
Speed to protein
For important research, speed is a critical need. The Expi293 PRO system's fast expression kinetics allow you to produce proteins in significantly less time (2–7 days) and with fewer steps, streamlining the research and development processes. This rapid turnaround is essential for meeting tight project timelines, advancing research goals, and bringing new discoveries to market faster.
Figure 9. Expi293 PRO system reaches up to 5 g/L titers within 7 days post-transfection while maintaining robust cell growth and viability.
High throughput and automation compatible
The Expi293 PRO system is designed for scalability, featuring simplified protocols and optimized reagents that seamlessly integrate with small-scale and automated platforms. This compatibility enables high-throughput protein production, allowing researchers to efficiently manage large volumes of samples and data. By supporting automation, the Expi293 PRO system enhances productivity and helps ensure consistent, reliable results across diverse applications.
Figure 10. Expi293 PRO system enables simplified, scalable transfection workflows in 96-well format—an ideal choice for automated expression screening and small-scale production.
Figure 11. Expi293 PRO system achieves consistently high titers across small scales in automated platforms.
More sustainable
Thermo Fisher Scientific is committed to developing our products with sustainability in mind. The Expi293 PRO system is not only more efficient but also more sustainable offering less waste, responsible packaging, and more energy efficiency. By achieving higher titers with less reagents, plasticware, and labor, researchers can significantly reduce their environmental footprint. This supports greener research practices and contributes to a greener future, making the Expi293 PRO system a more responsible choice for laboratories dedicated to both scientific excellence and environmental stewardship.
Table 1. Comparison of consumables needed for three expression systems.
System |
DIY PEI (polyethyleneimine) |
Expi293 System |
Expi293 PRO System |
|---|---|---|---|
Typical titers (IgG) |
10–200 mg/L |
200–1,000 mg/L |
800–5,000 mg/L |
Fold change |
N/A |
~4-fold |
~4-fold |
Media required |
~16 L |
~4 L |
~1 L |
Media bottles |
16 |
4 |
1 |
Shipping cardboard |
16 boxes |
4 boxes |
1 box |
Clarification filters |
16 |
4 |
1 |
Purification volume |
16 L |
4 L |
1 L |
Vessels |
1 x 50 L rocker bag or 6 x 5 L flasks |
2 x 5 L flasks |
1 x 3 L flask |
Expi293 PRO customer data and feedback
The Expi293 PRO Expression System is revolutionizing protein research and production, delivering excellent yields, efficiency, and versatility. But don't just take our word for it—see the real data and results, directly from scientists in your community, demonstrating the significant impact the Expi293 PRO system has had on the field.
Figure 12. In customer hands, the Expi293 PRO system increased expression of a wide variety of proteins by an average of 6x versus the Expi293 system.
Figure 13. Expi293 PRO system enabled 5–10x higher expression of SARS-CoV-2 spike proteins, including difficult Omicron variants, compared to the Expi293 system. Work completed by Dominic Esposito’s team at the Frederick National Laboratory for Cancer Research.
Order Expi293 PRO Expression System
Product |
Sizes |
Cat. No. |
Expi293 PRO System Kit |
1 kit |
|
Expi293 PRO Cells |
1 vial 6 vials |
|
Expi293 PRO Expression Medium |
1 L 6 x 1 L 10 L 20 L |
|
Expi293 PRO Transfection Kit
|
For 1 L transfection For 6 L transfection For 10 L transfection For 50 L transfection |
Need to extend your capacity?
The Expi293 PRO Expression System is also available for GeneArt Protein Expression Services so you can flex between in-house and outsourced protein production while maintaining consistency.
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