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Invitrogen
Phosphotyrosine Monoclonal Antibody (pY20), Sepharose® 4B
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Product Details
03-7742
Applications
Tested Dilution
Publications
Product Specifications
Published species
Chemical
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
pY20
Immunogen
Keyhole limpet hemocyanin coupled with equal amounts of phosphotyrosine, glycine, and alanine
Conjugate
Sepharose® 4B
Sepharose® 4B
Sepharose® 4B
Form
Slurry
Storage conditions
4°C, do not freeze
Shipping conditions
Wet ice
RRID
Product Specific Information
This monoclonal antibody is highly purified and then conjugated to agarose (4% cross-linked). The conjugate is supplied as 0.5 mL of settled gel in 1 mL of 50% suspension containing 10mM PBS, pH 7.0, 100mM NaCI, and 0.1% proclin.
This antibody is known to show reactivity towards phosphothreonine.
Target Information
The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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