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          • Primary Antibodies ›
          • SRC Antibodies

          Invitrogen

          Phospho-SRC (Tyr418) Monoclonal Antibody (SC1T2M3), PE, eBioscience™

          1 Reference
          View all (71) SRC antibodies

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          Cite Phospho-SRC (Tyr418) Monoclonal Antibody (SC1T2M3), PE, eBioscience™

          Additional Information:
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          • Antibody Testing Data (1)
          Phospho-SRC (Tyr418) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          Phospho-SRC (Tyr418) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Phospho-SRC (Tyr418) Antibody (12-9034-42) in Flow

          Intracellular staining of mouse spleen cells untreated (orange histogram) or treated with staurosporine for 2 hours (purple histogram) with Anti-Human/Mouse phospho-Src (Y418) PE using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Phospho-SRC (Tyr418) Antibody in Flow Cytometry (Flow)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          12-9034-42

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          5 µL (0.25 µg)/test
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Mouse

          Host/Isotype

          Mouse / IgG2b, kappa

          Recommended Isotype Control

          Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          SC1T2M3

          Conjugate

          PE PE PE
          • Unconjugated
          • eFluor 660
          • PerCP-eFluor 710
          • Request custom conjugation

          Excitation/Emission Max

          565/576 nm View spectra spectra

          Form

          Liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2572681

          Product Specific Information

          Description: This SC1T2M3 monoclonal antibody recognizes human and mouse Src tyrosine kinase (also known as ASV, c-src, c-SRC, p60-Src, pp60c-src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, SRC1) when phosphorylated on tyrosine 418 (Y418). Autophosphorylation of Src at Y418 in the catalytic domain is required for full catalytic activity of this kinase. Src is a non-receptor tyrosine kinase involved in signal transduction in numerous biological systems and is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. Aberrant Src activity has been implicated in the development of numerous types of cancer. Due to the sequence homology surrounding Src Y418, this SC1T2M3 clone is predicted to cross-react with many Src family kinases including Src, Lck, Fyn, and Lyn.

          Specificity of this SC1T2M3 clone was determined by ELISA, flow cytometry, and western blotting.

          Applications Reported:This SC1T2M3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

          Applications Tested: This SC1T2M3 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the BestProtocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the BestProtocols® Section under the Resources tab online.

          Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: EC 2.7.10.2; neuronal proto-oncogene tyrosine-protein kinase Src; OTTHUMP00000174476; OTTHUMP00000174477; OTTHUMP00000174478; p60-Src; pp60src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; Rous sarcoma oncogene; RP5-823N20.1; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; tyrosine-specific protein kinase; unnamed protein product; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

          View more View less

          Gene Aliases: ASV; c-SRC; p60-Src; pp60c-src; SRC1; THC6

          View more View less

          UniProt ID: (Human) P12931, (Mouse) P05480

          View more View less

          Entrez Gene ID: (Human) 6714, (Mouse) 20779

          View more View less

          Function(s)
          protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity receptor binding integrin binding protein binding ATP binding phospholipase activator activity kinase activity enzyme binding protein domain specific binding heme binding protein tyrosine kinase activator activity receptor activator activity ionotropic glutamate receptor binding SH2 domain binding phospholipase binding ion channel binding cadherin binding ephrin receptor binding ATPase binding phosphoprotein binding BMP receptor binding connexin binding scaffold protein binding protein kinase C binding insulin receptor binding protein kinase binding estrogen receptor binding macromolecular complex binding cell adhesion molecule binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter primary ovarian follicle growth positive regulation of cytokine production protein phosphorylation cell adhesion epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway gamete generation lactation positive regulation of cell proliferation positive regulation of gene expression negative regulation of gene expression regulation of epithelial cell migration positive regulation of epithelial cell migration positive regulation of glucose metabolic process positive regulation of protein processing positive regulation of smooth muscle cell migration cell migration protein deubiquitination peptidyl-tyrosine phosphorylation regulation of cell-cell adhesion cell differentiation positive regulation of cell migration forebrain development negative regulation of protein complex assembly protein destabilization cellular response to nutrient levels negative regulation of telomere maintenance regulation of intracellular estrogen receptor signaling pathway toll-like receptor 3 signaling pathway regulation of toll-like receptor 3 signaling pathway substrate adhesion-dependent cell spreading cellular response to reactive oxygen species positive regulation of Rac protein signal transduction positive regulation of dephosphorylation negative regulation of hippo signaling intracellular signal transduction osteoclast development cellular response to platelet-derived growth factor stimulus peptidyl-tyrosine autophosphorylation angiotensin-activated signaling pathway TORC1 signaling vasodilation odontogenesis positive regulation of apoptotic process negative regulation of apoptotic process stress fiber assembly proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of MAP kinase activity transcytosis bone resorption positive regulation of Notch signaling pathway positive regulation of bone resorption positive regulation of cell adhesion positive regulation of cell size positive regulation of glycolytic process positive regulation of Ras protein signal transduction positive regulation of insulin receptor signaling pathway neurotrophin TRK receptor signaling pathway focal adhesion assembly oogenesis positive regulation of peptidyl-tyrosine phosphorylation progesterone receptor signaling pathway positive regulation of small GTPase mediated signal transduction positive regulation of protein transport negative regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of mitochondrial depolarization uterus development branching involved in mammary gland duct morphogenesis regulation of cell projection assembly intestinal epithelial cell development interleukin-6-mediated signaling pathway cellular response to hydrogen peroxide positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to peptide hormone stimulus cellular response to progesterone stimulus cellular response to fluid shear stress cellular response to transforming growth factor beta stimulus positive regulation of podosome assembly DNA biosynthetic process regulation of heart rate by cardiac conduction positive regulation of canonical Wnt signaling pathway positive regulation of wound healing positive regulation of protein localization to nucleus positive regulation of intracellular signal transduction negative regulation of neutrophil activation positive regulation of cellular protein localization negative regulation of TORC1 signaling positive regulation of TORC1 signaling cellular response to angiotensin positive regulation of vascular smooth muscle cell proliferation positive regulation of neural precursor cell proliferation positive regulation of male germ cell proliferation positive regulation of ovarian follicle development positive regulation of lamellipodium morphogenesis positive regulation of platelet-derived growth factor receptor-beta signaling pathway regulation of early endosome to late endosome transport negative regulation of anoikis negative regulation of extrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway regulation of caveolin-mediated endocytosis stimulatory C-type lectin receptor signaling pathway negative regulation of inflammatory response to antigenic stimulus signal transduction signal complex assembly macroautophagy platelet activation osteoclast differentiation T cell costimulation positive regulation of integrin activation Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway regulation of vascular permeability regulation of bone resorption viral entry into host cell vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway leukocyte migration
          It has to be done as per old AB suggested Products section.

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