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Invitrogen
Mouse IgD Monoclonal Antibody (11-26c (11-26)), Biotin, eBioscience™
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FIGURE: 1 / 4
Mouse IgD Antibody (13-5993-82) in IHC
Nature communications 2018 -
Fig. 1 Loss of miR-146a in B cells led to spontaneous GC reactions in aged mice. FACS analyses and frequencies of a PNA + GL7 + GC B cells and b CXCR5 + PD-1 + Tfh cells in spleen from ~6 months old B-KO mice or their WT littermates. c Immunohistological analyses of GC reactions in SRBC-immunized spleen that were cryocut and stained with CD4 (red), PNA (green), and IgD (blue). d ELISA analysis of ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-5993-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Fish,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
11-26c (11-26)
Conjugate
Biotin
Biotin
Biotin
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.
Applications Reported: The 11-26c (11-26) antibody has been reported for use in flow cytometric analysis to detect the surface IgD on mouse B cells.
Applications Tested: The 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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