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Invitrogen
E-cadherin Monoclonal Antibody (ECCD-2)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
.jpg?time=20220826?time=20220826 "E-cadherin Antibody (13-1900) in IHC")
.jpg?time=20220826?time=20220826 "E-cadherin Antibody (13-1900) in IHC")
.jpg?time=20220826?time=20220826 "E-cadherin Antibody (13-1900) in IHC")
Product Details
13-1900
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Avian,
Dog,
Fish,
Hamster,
Human,
Mouse,
Pig,
Rat,
Tag
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
ECCD-2
Immunogen
Mouse liver E-cadherin fragment.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
2.0 mg/mL
Amount
100 µg
Purification
Ion-exchange chromatography
Storage buffer
TBS, pH 7.5, with 1% BSA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Source: Monoclonal antibody was obtained by fusing the P3-X63-Ag8 mouse myeloma cell line with spleen cells of Donryu rat after immunization with mouse teratocarcinoma and mouse liver E-cadherin fragment.
Specificity: This antibody specifically reacts with mouse E-cadherin. This antibody does not inhibit E-cadherin-dependent cell-cell contact.
Cross reactivity: This antibody reacts with human and dog E-cadherin. This antibody does not react with bovine or chicken E-cadherin.
Western blot can be performed under reducing or non-reducing conditions.
Reconstitution and dilution: dissolve the lyophilized antibody in 50 µl of distilled water to a final concentration 2.0 mg/mL. This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution in 500 µL or more of the following dilution solution: 20mM TBS (pH 7.5), 10mM CaCl2, 1.0% BSA, 0.1% NaN3. Note that TBS should be used for dilution, as PBS causes precipitation.
Storage: When stored at 4°C, 0.1% sodium azide should be added as a preservative.Store the antibody at a minimum concentration of 2.0 mg/mL; a lower antibody concentration may result in decreased stability.The stock solution (2.0mg/mL) should be stored in aliquots at -20°C for 1year, or stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored.
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cadherin e; cadherin-E; E-cadherin; Ecadherin; epithelial cadherin; unnamed protein product; uvomorulin
Gene Aliases: ARC-1; E-cad; Ecad; L-CAM; Um; UVO
UniProt ID: (Mouse) P09803
Entrez Gene ID: (Mouse) 12550
calcium ion binding
protein binding
beta-catenin binding
cytoskeletal protein binding
protein phosphatase binding
protein domain specific binding
ankyrin binding
GTPase activating protein binding
identical protein binding
alpha-catenin binding
gamma-catenin binding
cadherin binding
cell adhesion molecule binding
binding, bridging
cell adhesion mediator activity
protein binding involved in cell-cell adhesion
protein tyrosine kinase binding
cell morphogenesis
in utero embryonic development
trophectodermal cell differentiation
desmosome assembly
epithelial cell morphogenesis
cell-cell junction assembly
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
synapse assembly
embryo implantation
sensory perception of sound
regulation of gene expression
negative regulation of protein processing
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
negative regulation of cell-cell adhesion
positive regulation of cell-cell adhesion
actin cytoskeleton organization
negative regulation of cell migration
negative regulation of axon extension
regulation of protein localization
cell junction assembly
adherens junction organization
uterine epithelium development
positive regulation of protein import into nucleus
cell-cell adhesion mediated by cadherin
positive regulation of transcription, DNA-templated
decidualization
negative regulation of epithelial cell proliferation
intestinal epithelial cell development
salivary gland cavitation
regulation of branching involved in salivary gland morphogenesis
establishment of skin barrier
bicellular tight junction assembly
cellular response to amino acid stimulus
cellular response to lithium ion
cellular response to indole-3-methanol
protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
cochlea development
cell-cell adhesion
regulation of protein catabolic process at postsynapse, modulating synaptic transmission
negative regulation of protein localization to plasma membrane
positive regulation of cellular protein localization
regulation of protein localization to cell surface
regulation of neuron migration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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