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Invitrogen
Bcl-2 Monoclonal Antibody (Bcl-2-100)
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FIGURE: 1 / 44
Bcl-2 Antibody (13-8800) in IHC (P)
Immunohistochemical analysis of Bcl-2 was performed using formalin-fixed paraffin-embedded human colon cancer (Diffuse large B cell lymphoma) tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
13-8800
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Bacteria,
Chicken,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
Bcl-2-100
Immunogen
Synthetic peptide corresponding to residues 41-54 of the human Bcl-2 protein. This sequence is not shared by the mouse Bcl-2 protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; bcl-2 protein; bcl2-alpha protein; bcl2-beta protein; bcl2-Ig fusion gene; OTTHUMP00000163680; protein phosphatase 1, regulatory subunit 50
Gene Aliases: AW986256; Bcl-2; BCL2; C430015F12Rik; D630044D05Rik; D830018M01Rik; PPP1R50
UniProt ID: (Mouse) P10417
Entrez Gene ID: (Human) 596, (Mouse) 12043
protease binding
protein binding
channel activity
channel inhibitor activity
protein phosphatase binding
ubiquitin protein ligase binding
identical protein binding
sequence-specific DNA binding
protein heterodimerization activity
BH3 domain binding
protein phosphatase 2A binding
binding, bridging
DNA-binding transcription factor binding
transcription factor binding
protein homodimerization activity
BH domain binding
protein polyubiquitination
release of cytochrome c from mitochondria
apoptotic process
humoral immune response
cellular response to DNA damage stimulus
female pregnancy
positive regulation of cell proliferation
extrinsic apoptotic signaling pathway via death domain receptors
intrinsic apoptotic signaling pathway in response to DNA damage
response to radiation
response to xenobiotic stimulus
response to toxic substance
response to iron ion
regulation of autophagy
negative regulation of autophagy
programmed cell death
positive regulation of cell growth
positive regulation of B cell proliferation
hair follicle morphogenesis
endoplasmic reticulum calcium ion homeostasis
response to cytokine
response to nicotine
B cell proliferation
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
pigmentation
regulation of neuron apoptotic process
negative regulation of neuron apoptotic process
regulation of viral genome replication
regulation of mitochondrial membrane permeability
B cell receptor signaling pathway
neuron apoptotic process
defense response to virus
regulation of mitochondrial membrane potential
negative regulation of mitochondrial depolarization
regulation of calcium ion transport
transmembrane transport
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
regulation of intracellular signal transduction
negative regulation of anoikis
regulation of apoptotic signaling pathway
negative regulation of apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of intrinsic apoptotic signaling pathway
cell morphogenesis
response to acid chemical
ossification
ovarian follicle development
metanephros development
ureteric bud development
branching involved in ureteric bud morphogenesis
behavioral fear response
leukocyte homeostasis
B cell homeostasis
kidney development
regulation of cell-matrix adhesion
lymphocyte homeostasis
lymphoid progenitor cell differentiation
B cell lineage commitment
T cell lineage commitment
immune system development
response to ischemia
renal system process
protein dephosphorylation
melanin metabolic process
regulation of nitrogen utilization
cellular calcium ion homeostasis
response to oxidative stress
actin filament organization
axonogenesis
cell aging
cell proliferation
negative regulation of cell proliferation
male gonad development
intrinsic apoptotic signaling pathway in response to oxidative stress
apoptotic mitochondrial changes
post-embryonic development
organ morphogenesis
response to UV-B
response to gamma radiation
regulation of gene expression
negative regulation of calcium ion transport into cytosol
regulation of glycoprotein biosynthetic process
mesenchymal cell development
positive regulation of neuron maturation
positive regulation of smooth muscle cell migration
cell growth
single organismal cell-cell adhesion
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
cochlear nucleus development
gland morphogenesis
regulation of transmembrane transporter activity
hemopoiesis
B cell differentiation
T cell differentiation
negative regulation of ossification
negative regulation of cell growth
melanocyte differentiation
negative regulation of cell migration
axon regeneration
regulation of protein stability
glomerulus development
negative regulation of cellular pH reduction
regulation of protein localization
negative regulation of myeloid cell apoptotic process
T cell differentiation in thymus
positive regulation of peptidyl-serine phosphorylation
negative regulation of osteoblast proliferation
organ growth
growth
positive regulation of multicellular organism growth
cellular response to glucose starvation
response to drug
response to hydrogen peroxide
T cell homeostasis
regulation of programmed cell death
positive regulation of catalytic activity
CD8-positive, alpha-beta T cell lineage commitment
regulation of protein homodimerization activity
regulation of protein heterodimerization activity
ear development
positive regulation of melanocyte differentiation
negative regulation of mitotic cell cycle
negative regulation of retinal cell programmed cell death
focal adhesion assembly
developmental pigmentation
regulation of developmental pigmentation
positive regulation of developmental pigmentation
spleen development
thymus development
response to steroid hormone
digestive tract morphogenesis
developmental growth
oocyte development
positive regulation of skeletal muscle fiber development
pigment granule organization
homeostasis of number of cells within a tissue
regulation of catalytic activity
response to glucocorticoid
regulation of cell cycle
cellular response to organic substance
cellular response to hypoxia
reactive oxygen species metabolic process
negative regulation of G1/S transition of mitotic cell cycle
negative regulation of reactive oxygen species metabolic process
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