For superior cDNA synthesis performance upgrade to Invitrogen™ SuperScript™ IV Reverse Transcriptase.

      SuperScript™ II Reverse Transcriptase

      SuperScript™ II Reverse Transcriptase

      Numéro de catalogue:  18064022

      Invitrogen™  Applications liées: Reverse Transcription

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      Description

      Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA.

      SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.

      Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.

      For Research Use Only. Not for use in diagnostic procedures.

      Spécifications

      Optimal Reaction Temperature: 42° C
      Final Product Size(s): 12.3 kb or less
      Reverse Transcriptase: SuperScript™ II
      Ribonuclease H Activity: Reduced
      Concentration: 200 U⁄µl
      Enzyme Function: RNA dependent DNA Polymerase
      Form: Frozen
      Format: Tube(s)
      Number of Reactions: 10 Reactions
      Product Line: SuperScript™
      Product Size: 2,000 units
      Quantity: 2000 units
      Shipping Condition: Wet Ice

      Contenu et stockage

      SuperScript™ II Reverse Transcriptase (2,000 units total, at 200U/µL) is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.

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