Informational:For superior cDNA synthesis performance upgrade to Invitrogen™ SuperScript™ IV Reverse Transcriptase.
Invitrogen™

SuperScript™ II Reverse Transcriptase

Catalog number: 18064022
Invitrogen™

SuperScript™ II Reverse Transcriptase

Catalog number: 18064022
Catalog Number
18064022
Unit Size
2,000 units
Price (USD)
Price: 116.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
4 x 10,000 units
Price (USD)
Price: 1,416.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
10,000 units
Price (USD)
Price: 412.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
180640222,000 units
Price: 116.00
Your Price:
***
180640714 x 10,000 units
Price: 1,416.00
Your Price:
***
1806401410,000 units
Price: 412.00
Your Price:
***
Product Overview
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Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA.

SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.

Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
200 U/μL
Final Product Type
cDNA (First-Strand)
Form
Frozen
Format
Stand-alone enzyme
GC-Rich PCR Performance
High
No. of Reactions
10 Reactions
PCR Method
RT-PCR, RT-qPCR
Reaction Format
Separate components
Reaction Speed
Standard
Reagent Type
Reverse Transcription
Sample Type
RNA
Size (Final Product)
12.3 kb or Less
Starting Material
RNA
Technique
Reverse Transcription
Volume
10 μL
Enzyme Function
RNA dependent DNA Polymerase
Includes
SuperScript II Reverse Transcriptase (2,000 units total, at 200U/μL), 5X first-strand buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl2], and 100mM DTT
No. of Tests
10 Reactions
Optimal Reaction Temperature
42°C
Product Line
SuperScript™
Product Type
Reverse Transcriptase
Quantity
2000 units
Reverse Transcriptase
SuperScript™ II
Ribonuclease H Activity
Reduced
Shipping Condition
Wet Ice
Sufficient For
10 Reactions

Contents & Storage

SuperScript™ II Reverse Transcriptase (2,000 units total, at 200U/μL) is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.