Thermo Scientific™

Sulfo-LC-SDA (Sulfo-NHS-LC-Diazirine) (sulfosuccinimidyl 6-(4,4'-azipentanamido)hexanoate)

Catalog number: 26174
Thermo Scientific™

Sulfo-LC-SDA (Sulfo-NHS-LC-Diazirine) (sulfosuccinimidyl 6-(4,4'-azipentanamido)hexanoate)

Catalog number: 26174
Catalog Number
26174
Unit Size
50 mg
Price (USD)
Price: 459.00
Online price: 448.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
2617450 mg
Price: 459.00
Online price: 448.65
Your price:
-
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Thermo Scientific Pierce Sulfo-LC-SDA (Sulfo-NHS-LC-Diazirine) combines proven NHS-ester and diazirine-based photoreaction chemistries with conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation. A 12.5Å spacer arm separates the two photoreactive groups.

Features of Sulfo-LC-SDA (Sulfo-NHS-LC-Diazirine):

Water soluble—solubility in aqueous solutions improved by a sulfonate group
Heterobifunctional—NHS ester group reacts with primary amines at pH 7 to 9 to form covalent amide bonds; diazirine (azipentanoate) group reacts efficiently with any amino acid side chain or peptide backbone upon activation with long-wave UV light (330-370 nm)
Controllable—two-step chemical crosslinking is activated using common laboratory UV lamps
Easy to use—these crosslinkers are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark
Better than aryl azides—the diazirine photoreactive group has better photostability in normal light than phenyl azide groups of traditional photoreactive crosslinkers, yet the diazirine group is more efficiently activated by long-wave UV light

Succinimidyl-diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions, and these SDA reagents extend the efficiency and range of interactions that can be explored by this approach.

Typical bifunctional amine- or sulfhydryl-reactive crosslinkers require specific amino acid groups (e.g., lysine or cysteine) at the correct distance on both proteins to capture protein interactions. The SDA crosslinkers circumvent this limitation by allowing specific labeling of one protein using the amine-reactive N-hydroxysuccinimide (NHS) ester followed by UV-activated crosslinking via the diazirine moiety to any amino acid side chain or peptide backbone of a second protein. Diazirine-based photocrosslinkers have better photostability than phenyl azide-based photocrosslinkers and are easily activated with long-wave UV light (330-370nm).

Multiple Diazirine Crosslinkers Available
The NHS-ester diazirine derivatives (SDA, LC-SDA and SDAD) lack a charged group and are membrane-permeable. This property makes them ideal for intracellular and intramembrane conjugations. By contrast, Sulfo-SDA, Sulfo-LC-SDA and Sulfo-SDAD contain negatively charged sulfate groups that improve their water solubility and reduce cell membrane permeability, enabling their use for extracellular protein crosslinking. SDAD and Sulfo-SDAD also have a disulfide bond within the spacer that can be cleaved with reducing agents.

• Short (3.9Å) and long (12.5Å) spacer arm varieties
• Membrane-permeable (NHS) and impermeable (Sulfo-NHS) varieties
• Non-cleavable and cleavable (disulfide spacer) varieties

Related Products
LC-SDA (NHS-LC-Diazirine)
Sulfo-SDA (Sulfo-NHS-Diazirine)
Sulfo-SDAD (Sulfo-NHS-SS-Diazirine)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Reactivity
Amine-Nonselective
Cleavable
No
Description
Sulfo-LC-SDA
Molecular Weight (g/mol)
440.4
PEGylated
No
Spacer Arm Length
12.5 Å
Cell Permeability
No
Shipping Condition
Ambient
Product Line
Pierce™
Labeling Method
Chemical Labeling
Crosslinker Type
Heterobifunctional
Reactive Moiety
Diazirine, Sulfo-NHS Ester
Spacer
Medium (10 to 30 Å)
Form
Solid
Quantity
50 mg
Solubility
Water
Format
Standard
Water Soluble
Yes
Product Type
Crosslinker

Contents & Storage

Upon receipt store product at 4°C protected from light and moisture.

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