Pierce™ Recombinant Protein L, Biotinylated

Catalog number: 29997

Thermo Scientific™  Related applications: Cellular Imaging | Protein Assays and Analysis

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Use Thermo Scientific Pierce Biotinylated Recombinant Protein L to probe and detect mouse and human antibodies containing kappa light chains in Western blotting, ELISA, IHC and other immunoassay protocols.

Features of Recombinant Protein L:

• Contains four immunoglobulin-binding domains per protein
• Binds members of all classes of Ig (IgG, IgM, IgA, IgE and IgD) that contain kappa light chains
• Binds single chain variable fragments (scFv) and Fab fragments that contain kappa light chains

Protein L is an immunoglobulin-binding protein that was originally derived from the bacteria Peptostreptococcus magnus, but is now produced recombinantly. Protein L has the unique ability to bind through kappa light chain interactions without interfering with an antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L will bind to all classes of Ig (IgG, IgM, IgA, IgE and IgD). Protein L will also bind single chain variable fragments (scFv) and Fab fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.

Properties of Recombinant Protein L:
• Source: E. coli
• Molecular Weight: ~35,800 (Apparent MW by SDS-PAGE: 36,000)
• Form: salt-free powder
• A280 of 0.1% solution: ~0.80
• Isoelectric point (pI): 4.8

Related Products
Pierce™ Recombinant Protein L
Pierce™ Recombinant Protein L, Peroxidase Conjugated
For Research Use Only. Not for use in diagnostic procedures.


Label or Dye: Biotin
Product Size: 0.5 mg
Target Molecule: Antibodies

Contents & storage

Upon receipt store at -20°C. Product is shipped at ambient temperature.


Manuals & protocols

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