Applied Biosystems™

TaqMan™ SNP Genotyping Assay, mouse This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  4351384

Related applications:

Real Time PCR (qPCR)

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Description

Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5´‑nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP), and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). One probe is labeled with VIC dye to detect the Allele 1 sequence; the second probe is labeled with FAM dye to detect the Allele 2 sequence.

The predesigned TaqMan SNP Genotyping Mouse Assay collection consists of over 10,000 assays. For targets of interest that are not covered by the predesigned assay collection, Custom TaqMan SNP Genotyping Assays are available to meet your research needs.

Benefits:
Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
Guaranteed—if you are not satisfied with the performance of a TaqMan Assay, we’ll replace it at no cost, or credit your account*

Approximate ship time
4–6 days in North America and 6–10 days in Europe

TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately).

All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*

Recommended master mix (sold separately): TaqMan Genotyping Master Mix

*Terms and conditions apply. For complete details, go to www.thermofisher.com/taqmanguarantee.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

3'Primer Modification: None
5'Primer Modification: None
CTA Type: Magellan_PDP_OrderNow
Green Features: Sustainable packaging
Internal Probe Modification: VIC® (5in), FAM (5in), MGB (Minor Groove Binder) (3in)
Label or Dye: FAM, VIC
Product Line: TaqMan™
Purification Method: Solid Phase Extraction (SPE)
Purity or Quality Grade: RNase-Free, DNase-Free
Remarks: VIC® - MGB probe detects Allele 1, FAM - MGB probe detects Allele 2
Shipping Condition: Room Temperature
Target Gene: Over 10,000 mouse assays are available
Concentration: 40 X
Form: Frozen
Species: Mouse

Contents & storage

1 tube containing a 40X (S and M sizes) or 80X (L size) mix of pre-formulated assay (2 probes and 2 primers).

Store at -15 to -25°C.