High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

Catalog number: 4374967

Applied Biosystems™  Related applications: Reverse Transcription

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. This particular kit also includes 2 tubes of RNase inhibitor (100 µL each).

The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

Features of this kit include:

• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• 10-fold greater dynamic range than other kits

Extensively tested with a variety of templates
Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.
For Research Use Only. Not for use in diagnostic procedures.


Reverse Transcriptase: MultiScribe™ MuLV
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Real-Time Quantitative PCR (qPCR)
Reaction Format: Separate Components
Sample Type (General): RNA
Product Size: 1,000 reactions
Final Product: cDNA (First-Strand)
Final Product Size(s): 7 kb or less
Optimal Reaction Temperature: 37° C

Contents & storage

• 10X RT Buffer (2 × 1 mL)
• 10X RT Random Primers (2 × 1 mL)
• 25X dNTP Mix (100 mM, 1 mL)
• MultiScribe® Reverse Transcriptase (1 mL at 50 U/µL)
• RNase Inhibitor (10 × 0.1 mL at 20 U/µL)

Store between -15°C and -25°C.


Manuals & protocols

Recommended products