Applied Biosystems™

High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor

Catalog number:  4374967

Related applications:

Reverse Transcription

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Description

The High Capacity cDNA Reverse Transcription Kit with RNase Inhibitor contains all components necessary for the quantitative conversion of up to 2 µg of total RNA to single-stranded cDNA in a single 20 µL reaction. This particular kit also includes 5 tubes of RNase inhibitor (200 µL each).

The High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to generate 10 µg of cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and microarrays. The kit is ideal for generating cDNA archives.

Features of this kit include:

• Linear target amplification for real-time PCR
• Higher yields and precision than other cDNA synthesis kits at a fraction of the cost
• 10-fold greater dynamic range than other kits

Extensively tested with a variety of templates
Quantitative first-strand synthesis of all RNA species is achieved with the use of the random primers. The kit has been extensively tested with a variety of RNA templates, including GC- and AU-rich targets and RNAs expressed at low levels. cRNA can also be efficiently generated from in vitro transcription of cDNA.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product Type: cDNA (First-Strand)
Includes: 10X RT Buffer (2 × 1mL); 10X RT Random Primers (2 × 1mL); 25X dNTP Mix (100mM, 1mL); MultiScribe Reverse Transcriptase (1mL at 50U/μL); RNase Inhibitor (10 × 0.1mL at 20U/μL)
Optimal Reaction Temperature: 37° C
Quantity: 1000 reactions
Reverse Transcriptase: MultiScribe™
Sample Type: RNA
Concentration: 25 X
Detection Method: Real Time PCR
Fidelity (vs. Taq): 25 X
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
Format: Tube
GC-Rich PCR Performance: High
No. of Reactions: 1000 Reactions
No. of Tests: 1000 Reactions
Product Type Specs: High-Capacity cDNA Reverse Transcription Kit
Reaction Format: Separate Components
Reaction Speed: Standard
Size (Final Product): 7 kb or less
Sufficient For: 1000 Reactions
Volume: 100 μL, 20 μL

Contents & storage

Contents:
• 10X RT Buffer (2 × 1 mL)
• 10X RT Random Primers (2 × 1 mL)
• 25X dNTP Mix (100 mM, 1 mL)
• MultiScribe™ Reverse Transcriptase (1 mL at 50 U/µL)
• RNase Inhibitor (5 × 0.2 mL at 20 U/µL)

Store between -15°C and -25°C.