Applied Biosystems™

TaqMan™ MicroRNA Assay

Green features
Catalog number: 4427975
Applied Biosystems™

TaqMan™ MicroRNA Assay

Green features
Catalog number: 4427975
Catalog Number
4427975
Unit Size
S (50 RT/150 PCR reactions), Inventoried
Price (USD)
232.00
Your Price:
Catalog Number
Unit Size
M (750 RT/750 PCR reactions), Made to Order
Price (USD)
854.00
Your Price:
Catalog Number
Unit Size
S (50 RT/150 PCR reactions), Made to Order
Price (USD)
358.00
Your Price:
Catalog Number
Unit Size
L (2900 RT/2900 PCR reactions), Made to Order
Price (USD)
1,870.00
Your Price:
Catalog Number
Unit Size
XS (25 RT/75 PCR reactions), Made to Order
Price (USD)
185.00
Your Price:
Catalog NumberUnit SizePrice (USD)
4427975S (50 RT/150 PCR reactions), Inventoried
232.00
Your Price:
4440887M (750 RT/750 PCR reactions), Made to Order
854.00
Your Price:
4440886S (50 RT/150 PCR reactions), Made to Order
358.00
Your Price:
4440888L (2900 RT/2900 PCR reactions), Made to Order
1,870.00
Your Price:
4440885XS (25 RT/75 PCR reactions), Made to Order
185.00
Your Price:
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes. TaqMan MicroRNA Assays are:

Highly specific—quantitate only mature miRNAs, not precursors
Sensitive—conserve limited samples, requires only 1–10 nanograms of total RNA or equivalent
Fast, simple and scalable—two-step quantitative RT-PCR assay provides high-quality results in less than three hours

Our TaqMan MicroRNA Assay catalogue is kept up to date with the Sanger database to provide comprehensive coverage with the most current annotation.

Availability and sizes
Made-to-Order assays are available in four different sizes and usually ship within two weeks. Inventoried assays are prepackaged in Small size and are ready to ship. Inventoried assays may be purchased in larger sizes by ordering them as Made to Order.

Species
TaqMan MicroRNA Assays are available for a range of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. We will continue to increase the number of miRNA assays for these species in alignment with the Sanger miRBase Registry.

Assay Components
TaqMan MicroRNA Assays are delivered in two tubes: one containing the RT primer, the other containing the specific pre-formulated TaqMan Assay (TaqMan probe and PCR primer set).

TaqMan miRNA Assay selection guide

 TaqMan MicroRNA AssaysTaqMan Advanced miRNA Assays
DescriptionTaqMan MicroRNA Assays employ a novel target-specific stem–loop primer during cDNA synthesis to produce a template for real-time PCRTaqMan Advanced miRNA Assays employ a universal RT step for a streamlined workflow, and a universal miR-Amp step to enable highly sensitive detection by real-time PCR
RT chemistrymiRNA-specific RTUniversal RT
ThroughputBest for 1–10 targetsBest for >10 targets
Coverage205 species available, coverage for miRBase v.21All human, mouse, and rat miRNAs; coverage for miRBase v.21
For Research Use Only. Not for use in diagnostic procedures.

Specifications

CTA Type
Magellan_PDP_OrderNow
Generic Product
TaqMan miRNA
Green Features
Sustainable packaging
No. of Tests
150 Reactions
Product Line
TaqMan™
Product Type
Configurable Assay
Shipping Condition
Room Temperature
Size
S (50 RT/150 PCR reactions), Inventoried
Sufficient For
150 Reactions
Format
Tube
GC-Rich PCR Performance
High
No. of Reactions
150 Reactions
PCR Method
qPCR, RT-PCR
Reaction Speed
Fast

Contents & Storage

• 1 tube containing RT primer at concentration of 5X (XS and S sizes), 20X (M size), or 60X (L size)
• 1 tube containing a 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15 to -25°C.

Figures

Frequently asked questions (FAQs)

Citations & References