Applied Biosystems™

TaqMan™ Mutation Detection Assay with castPCR™ Technology

Catalog number: 4465804
Have Questions?
Applied Biosystems™

TaqMan™ Mutation Detection Assay with castPCR™ Technology

Catalog number: 4465804
Have Questions?
Catalog Number
4465804
Unit Size
Mutant Allele (75 reactions), Inventoried
Price (USD)
Catalog Number
Unit Size
Gene Reference (75 reactions), Inventoried
Price (USD)
Catalog NumberUnit SizePrice (USD)
4465804Mutant Allele (75 reactions), Inventoried
4465807Gene Reference (75 reactions), Inventoried
Product Overview
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Applied Biosystems TaqMan Mutation Detection Assays are powered by competitive allele-specific TaqMan castPCR technology to detect and measure somatic mutations in genes associated with cancer research. castPCR technology is highly specific and sensitive, and can detect rare amounts of mutated DNA in a sample that contains large amounts of normal, wild-type DNA. These assays are compatible with different sample types, such as cell lines, formalin-fixed paraffin-embedded (FFPE) tissue samples, and fresh-frozen tissue samples.

castPCR technology
Competitive, allele-specific TaqMan PCR utilizes an allele-specific primer for mutant allele detection that competes with an MGB blocker oligonucleotide to suppress the wild-type background.

Somatic mutation detection
By combining the power of TaqMan and castPCR technologies, TaqMan Mutation Detection Assays provide:

Higher specificity—designed to block the wild-type and amplify only the mutant
Higher sensitivity—designed to detect somatic mutations down to 1 cancer cell in 1,000 normal cells
Faster workflow—3 hours from sample to result

With 819 assays for 47 known cancer research genes available today like KRAS, BRAF, KIT, JAK2, the TaqMan Mutation Detection Assays provide an innovative tool for your cancer research.

All TaqMan Mutation Detection Assays have undergone extensive testing to ensure verify high sensitivity and specificity.

Specifications

Detection Method
Primer-probe
Form
Frozen
Format
Tube
GC-Rich PCR Performance
High
PCR Method
qPCR
Reaction Speed
Fast
Product Line
TaqMan™
Product Type
Mutation Detection Assay
Quantity
Mutant Allele (75 reactions), Inventoried
Shipping Condition
Room Temperature
Concentration
10X
Fidelity (vs. Taq)
10 X

Contents & Storage

1 tube containing a 10X mix of unlabeled PCR primers, a TaqMan MGB probe, and an MGB oligonucleotide blocker.

Store at -15 to -25°C.

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