Applied Biosystems™

TaqMan™ Mutation Detection Assay (castPCR)

Catalog number:  4465804

 Related applications:

Real Time PCR (qPCR)

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TaqMan Mutation Detection Assays are powered by competitive allele-specific TaqMan PCR (castPCR) technology to detect and measure somatic mutations in genes associated with cancer research. castPCR technology is highly specific and sensitive, and can detect rare amounts of mutated DNA in a sample that contains large amounts of normal, wild-type DNA. These assays are compatible with different sample types, such as cell lines, formalin-fixed paraffin-embedded (FFPE) tissue samples, and fresh-frozen tissue samples.

castPCR technology
Competitive, allele-specific TaqMan PCR utilizes an allele-specific primer for mutant allele detection that competes with an MGB blocker oligonucleotide to suppress the wild-type background.

Somatic mutation detection
By combining the power of TaqMan and castPCR technologies, TaqMan Mutation Detection Assays provide:

Higher specificity—designed to block the wild-type and amplify only the mutant
Higher sensitivity—designed to detect somatic mutations down to 1 cancer cell in 1,000 normal cells
Faster workflow—3 hours from sample to result

With 819 assays for 47 known cancer research genes available today like KRAS, BRAF, KIT, JAK2, the TaqMan Mutation Detection Assays provide an innovative tool for your cancer research.

All TaqMan Mutation Detection Assays have undergone extensive testing to ensure verify high sensitivity and specificity.


Product Line: TaqMan™
Product Size: Mutant Allele (75 reactions), Inventoried
Shipping Condition: Room Temperature

Contents & storage

1 tube containing a 10X mix of unlabeled PCR primers, a TaqMan MGB probe, and an MGB oligonucleotide blocker.

Store at -15 to -25°C.


Manuals & protocols