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Invitrogen

IL-1 beta (Pro-form) Monoclonal Antibody (NJTEN3), PerCP-eFluor™ 710, eBioscience™

28 Published Figures
26 References
View all (7) IL-1 beta (Pro-form) antibodies

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Product Details

46-7114-82

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.25 µg/test
View 26 publications 26 publications
Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

NJTEN3

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573835

Product Specific Information

Description: This NJTEN3 monoclonal antibody reacts with the pro-form of mouse IL-1 beta, which is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is instead secreted alongside caspase-1 via an alternate and incompletely understood mechanism. IL-1 beta signals via the IL-1RI, which is shared with IL-1 alpha. These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1 beta neutralizing antibodies potential therapeutical value. The NJTEN3 antibody recognizes only the pro-form of mouse IL-1 beta and does not see the active (cleaved) form.

Applications Reported: This NJTEN3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This NJTEN3 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse thioglycolate-elicited peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is instead secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions.IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1 beta neutralizing antibodies potential therapeutical value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: il 1b; IL 1β; IL-1 beta; IL1β; ILN; Interleukin; interleukin 1b; Interleukin-1b; Interleukin1 beta; unnamed protein product

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Gene Aliases: Il-1b; IL-1beta

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UniProt ID: (Mouse) P10749

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Entrez Gene ID: (Mouse) 16176

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Function(s)
cytokine activity interleukin-1 receptor binding integrin binding protein domain specific binding receptor agonist activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter inflammatory response immune response positive regulation of cytosolic calcium ion concentration memory positive regulation of cell proliferation negative regulation of cell proliferation response to carbohydrate vascular endothelial growth factor production positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression positive regulation of platelet-derived growth factor receptor signaling pathway positive regulation of epithelial to mesenchymal transition positive regulation of macrophage derived foam cell differentiation negative regulation of glucose transport negative regulation of glutamate secretion cytokine-mediated signaling pathway hyaluronan biosynthetic process positive regulation of cell migration neutrophil chemotaxis positive regulation of prostaglandin biosynthetic process positive regulation of fever generation lipopolysaccharide-mediated signaling pathway positive regulation of prostaglandin secretion response to lipopolysaccharide positive regulation of chemokine production positive regulation of granulocyte macrophage colony-stimulating factor production positive regulation of interferon-gamma production positive regulation of interleukin-2 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of stress-activated MAPK cascade regulation of organic acid transport positive regulation of immature T cell proliferation in thymus response to ATP positive regulation of heterotypic cell-cell adhesion social behavior ectopic germ cell programmed cell death positive regulation of T cell proliferation positive regulation of apoptotic process regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of MAPK cascade positive regulation of MAPK cascade positive regulation of JUN kinase activity positive regulation of neuron apoptotic process positive regulation of nitric oxide biosynthetic process negative regulation of neuron differentiation positive regulation of glial cell differentiation positive regulation of angiogenesis negative regulation of lipid metabolic process positive regulation of mitotic nuclear division positive regulation of transcription, DNA-templated positive regulation of complement activation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade negative regulation of insulin receptor signaling pathway astrocyte activation positive regulation of astrocyte differentiation regulation of defense response to virus by host positive regulation of inflammatory response regulation of neurogenesis negative regulation of neurogenesis regulation of insulin secretion negative regulation of synaptic transmission defense response to Gram-positive bacterium leukocyte migration negative regulation of lipid catabolic process positive regulation of lipid catabolic process regulation of nitric-oxide synthase activity positive regulation of membrane protein ectodomain proteolysis positive regulation of protein kinase B signaling positive regulation of glial cell proliferation negative regulation of adiponectin secretion regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade monocyte aggregation interleukin-1-mediated signaling pathway response to interleukin-1 cellular response to lipopolysaccharide cellular response to mechanical stimulus cellular response to xenobiotic stimulus positive regulation of monocyte chemotactic protein-1 production positive regulation of neutrophil chemotaxis extrinsic apoptotic signaling pathway in absence of ligand cellular response to interleukin-17 positive regulation of p38MAPK cascade positive regulation of NIK/NF-kappaB signaling positive regulation of RNA biosynthetic process regulation of establishment of endothelial barrier negative regulation of secretion by cell negative regulation of gap junction assembly positive regulation of occluding junction disassembly negative regulation of branching morphogenesis of a nerve negative regulation of neural precursor cell proliferation positive regulation of T-helper 1 cell cytokine production negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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