Intracellular staining of 6-day Th17-polarized mouse splenocytes with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.06 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.06 µg of Anti-Mouse IL-17AF eFluor® 660 (right). Cells were treated for 5 hours with Cell Stimulation Cocktail plus protein transport inhibitors (Product # 00-4975-03) prior to harvest and staining. Cells in the lymphocyte gate were used for analysis.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Product Details

50-9171-80

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

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Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B8KN8R

Immunogen

Recombinant IL-17AF

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

25 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Ambient (domestic); Wet ice (international)

AB_2574327

Product Specific Information

Description: The B8KN8R monoclonal antibody reacts with mouse interleukin (IL)-17AF heterodimer. This antibody specifically reacts with this heterodimer and has no reactivity for IL-17A or IL-17F homodimers. IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.

Applications Reported: This B8KN8R antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This B8KN8R antibody has been tested by intracellular staining and flow cytometric analysis of Th17-polarized mouse splenocytes after restimulation with Cell stimulation Cocktail plus protein transport inhibitors, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocls: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resource Tab online. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cytokine; cytokine; IL-17F; cytotoxic T-lymphocyte-associated antigen 8; il 17; ILN; Interleukin; unnamed protein product

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Gene Aliases: Ctla-8; Ctla8; IL-17; IL-17A; IL-17F; Il17

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UniProt ID: (Mouse) Q62386, (Mouse) Q7TNI7

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Entrez Gene ID: (Mouse) 16171, (Mouse) 257630

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Function(s)

receptor binding cytokine activity protein binding protein homodimerization activity protein heterodimerization activity cytokine receptor binding cytokine binding

Process(es)

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IL-17AF Monoclonal Antibody (B8KN8R), eFluor™ 660, eBioscience™

IL-17AF Antibody (50-9171-80) in Flow

Product Details

50-9171-80

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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