Invitrogen

Actin (muscle) Monoclonal Antibody (HHF35), Alexa Fluor™ 488, eBioscience™

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5 References

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Antibody Testing Data (1)
Published Figures (1)

Actin (muscle) Antibody in Immunocytochemistry (ICC/IF)

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Actin (muscle) Antibody (53-6496-82) in ICC/IF

Immunocytochemistry on fixed and permeabilized C2C12 cells stained with 30 µg/mL Mouse IgG1 Alexa Fluor® 488 Isotype Control (left) or 30 µg/mL Anti-Actin (muscle) Alexa Fluor® 488 (right). Nuclei are stained with DAPI.

Actin (muscle) Antibody in Immunohistochemistry (IHC)

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Product Details

53-6496-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

30 µg/mL

Product Specifications

Species Reactivity

Bovine, Dog, Horse, Guinea pig, Human, Mouse, Non-human primate, Pig, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HHF35

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2574433

Product Specific Information

Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42 kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas.

The HHF35 antibody recognizes human, mouse, rat, bovine, canine and non-human primate actin.

Applications Reported: This HHF35 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This HHF35 antibody has been tested by immunocytochemistry and can be used at less than or equal to 30 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ACTA1; ACTA2; ACTB; ACTC; ACTG2; actin; actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; actin homolog; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha skeletal muscle; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, cytoplasmic 2; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; actin-like protein; alpha actin; alpha actin 1; alpha actin 2; alpha-actin; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; B-actin; beta actin; beta-actin FE-3; cardiac actin; cardiac alpha actin; cardiac muscle alpha actin 1; Cell growth-inhibiting gene 46 protein; cytoplasmic actin; cytoskeletal gamma-actin; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; GIG46; nemaline myopathy type 3; RP5-1068B5.2; skeletal alpha actin; skeletal muscle alpha-actin 1; smooth muscle alpha-actin; Smooth muscle gamma-actin; unnamed protein product; vascular smooth muscle alpha-actin

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Gene Aliases: 0610041G09Rik; a-SMA; AA959943; ACT; ACT-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; actin; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; AL023024; alphaSMA; ASD5; ASMA; CFTD; CFTD1; CFTDM; CMD1R; CMH11; CMYO2A; CMYO2B; CMYO2C; CMYP2A; CMYP2B; CMYP2C; DFNA20; DFNA26; E51; GIG46; HEL-176; LVNC4; MMIHS5; MPFD; NEM1; NEM2; NEM3; SHPM; SMalphaA; SMDYS; SMGA; VSCM; VSCM1

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Function(s)

nucleotide binding structural constituent of cytoskeleton protein binding ATP binding hydrolase activity myosin binding ADP binding protein kinase binding microfilament motor activity profilin binding ubiquitin protein ligase binding identical protein binding structural constituent of postsynaptic actin cytoskeleton ATPase activity molecular_function actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

Process(es)

muscle contraction positive regulation of gene expression skeletal muscle thin filament assembly skeletal muscle fiber development mesenchyme migration regulation of blood pressure response to virus vascular smooth muscle contraction glomerular mesangial cell development positive regulation of hepatic stellate cell migration positive regulation of hepatic stellate cell contraction positive regulation of ERK1 and ERK2 cascade cellular response to transforming growth factor beta stimulus juxtaglomerular apparatus development positive regulation of hepatic stellate cell activation actin filament organization response to xenobiotic stimulus actin filament-based movement actomyosin structure organization actin-myosin filament sliding negative regulation of apoptotic process response to ethanol cardiac myofibril assembly cardiac muscle tissue morphogenesis heart contraction cardiac muscle contraction cytoplasmic actin-based contraction involved in cell motility angiogenesis morphogenesis of a polarized epithelium axonogenesis positive regulation of cell migration maintenance of blood-brain barrier sarcomere organization cell motility regulation of stress fiber assembly regulation of focal adhesion assembly platelet aggregation cellular response to interferon-gamma positive regulation of wound healing postsynaptic actin cytoskeleton organization tight junction assembly regulation of transepithelial transport regulation of synaptic vesicle endocytosis protein localization to bicellular tight junction response to mechanical stimulus response to extracellular stimulus response to lithium ion cell growth skeletal muscle fiber adaptation response to steroid hormone metabolic process response to drug actin-mediated cell contraction retina homeostasis response to calcium ion biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Actin (muscle) Monoclonal Antibody (HHF35), Alexa Fluor™ 488, eBioscience™

Actin (muscle) Antibody (53-6496-82) in ICC/IF

Product Details

53-6496-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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