Immunocytochemistry on fixed and permeabilized C2C12 cells stained with 30 µg/mL Mouse IgG1 Alexa Fluor® 488 Isotype Control (left) or 30 µg/mL Anti-Actin (muscle) Alexa Fluor® 488 (right). Nuclei are stained with DAPI.

Actin (muscle) Antibody in Immunohistochemistry (IHC)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Product Details

53-6496-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

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Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

30 µg/mL

Product Specifications

Species Reactivity

Bovine, Dog, Horse, Guinea pig, Human, Mouse, Non-human primate, Pig, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HHF35

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Ambient (domestic); Wet ice (international)

AB_2574433

Product Specific Information

Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42 kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas.

The HHF35 antibody recognizes human, mouse, rat, bovine, canine and non-human primate actin.

Applications Reported: This HHF35 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This HHF35 antibody has been tested by immunocytochemistry and can be used at less than or equal to 30 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ACTA1; ACTA2; ACTB; ACTC; ACTG2; actin; actin alpha 1; actin alpha cardiac; actin homolog; actin, alpha 1, skeletal muscle; actin, alpha 2, smooth muscle, aorta; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; actin-like protein; alpha actin; alpha actin 1; alpha actin 2; alpha-actin; alpha-actin cardiac; alpha-actin-1; alpha-actin-2; alpha-actin-3; alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; B-actin; beta actin; beta-actin FE-3; cardiac actin; cardiac alpha actin; cardiac muscle alpha actin 1; cell growth-inhibiting gene 46 protein; cytoplasmic actin; cytoskeletal gamma-actin; epididymis luminal protein 176; gamma-2-actin; gamma-actin; gamma-enteric smooth muscle actin; GIG46; nemaline myopathy type 3; RP5-1068B5.2; skeletal alpha actin; skeletal muscle alpha-actin; skeletal muscle alpha-actin 1; smooth muscle alpha-actin; smooth muscle gamma-actin; unnamed protein product; vascular smooth muscle alpha-actin

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Gene Aliases: 0610041G09Rik; a-SMA; ACT; ACT-4; Act4; ACTA; Acta-2; ACTA1; ACTA3; ACTC; Actc-1; ACTE; ACTG; ACTGE; actin; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; Actvs; alphaSMA; ASD5; ASMA; CFTD; CFTD1; CFTDM; CMD1R; CMH11; CMYO2A; CMYO2B; CMYO2C; CMYP2A; CMYP2B; CMYP2C; DFNA20; DFNA26; E51; HEL-176; LVNC4; MMIHS5; MPFD; NEM1; NEM2; NEM3; SHPM; SMAalpha; SMalphaA; SMDYS; SMGA; VSCM; VSCM1

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Function(s)

structural constituent of cytoskeleton protein binding ATP binding ATPase activity myosin binding ADP binding protein kinase binding microfilament motor activity profilin binding ubiquitin protein ligase binding identical protein binding structural constituent of postsynaptic actin cytoskeleton actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

Process(es)

muscle contraction positive regulation of gene expression skeletal muscle thin filament assembly skeletal muscle fiber development mesenchyme migration regulation of blood pressure response to virus vascular smooth muscle contraction glomerular mesangial cell development cytoskeleton organization positive regulation of hepatic stellate cell migration positive regulation of hepatic stellate cell contraction positive regulation of ERK1 and ERK2 cascade cellular response to transforming growth factor beta stimulus juxtaglomerular apparatus development positive regulation of hepatic stellate cell activation actin filament organization actin filament-based movement actomyosin structure organization actin-myosin filament sliding cardiac myofibril assembly cardiac muscle tissue morphogenesis heart contraction angiogenesis morphogenesis of a polarized epithelium axonogenesis positive regulation of cell migration maintenance of blood-brain barrier cell motility regulation of stress fiber assembly regulation of focal adhesion assembly platelet aggregation positive regulation of wound healing postsynaptic actin cytoskeleton organization tight junction assembly regulation of transepithelial transport regulation of synaptic vesicle endocytosis protein localization to bicellular tight junction response to ethanol cellular response to interleukin-6 cellular response to acetaldehyde response to mechanical stimulus cellular response to potassium ion skeletal muscle fiber adaptation response to steroid hormone response to xenobiotic stimulus negative regulation of apoptotic process cardiac muscle contraction cytoplasmic actin-based contraction involved in cell motility sarcomere organization cellular response to interferon-gamma response to calcium ion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Actin (muscle) Monoclonal Antibody (HHF35), Alexa Fluor™ 488, eBioscience™

Actin (muscle) Antibody (53-6496-82) in ICC/IF