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Invitrogen

CD16/CD32 Monoclonal Antibody (93), Alexa Fluor™ 700, eBioscience™

18 Published Figures
44 References
View all (56) CD16/CD32 antibodies

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Product Details

56-0161-80

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

-
View 2 publications 2 publications

Flow Cytometry (Flow)

0.25 µg/test
View 41 publications 41 publications

Neutralization (Neu)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse

Published species

Fruit fly, Human, Mouse

Host/Isotype

Rat / IgG2a, lambda

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

93

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra spectra

Form

Liquid

Concentration

0.2 mg/mL

Amount

25 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_493995

Product Specific Information

Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported: This 93 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: CD16A; CD16B; CD32 receptor 2; CDw32; fc gamma receptor IIB; Fc gamma receptor III; Fc gamma receptor precursor; Fc gamma RIIB; fc-gamma RII; Fc-gamma RII-b; Fc-gamma RII-c; fc-gamma RIII; Fc-gamma-1/gamma-2bR receptor precursor; Fc-gamma-R-alpha receptor; Fc-gamma-R-beta-1 precursor; Fc-gamma-R-beta-2 precursor; Fc-gamma-RII; fc-gamma-RIIB; Fc-gamma-RIIc; Fcg receptor III; FCG2; FcgammaRIIB; FcgammaRIII; FCGR2C; FCGR3A; FCGR3B; FCGRIII; FCR-10; FcRII-b; FcRII-c; fcRIII; FCRIIIA; FGFR2B; igG Fc receptor II beta; igG Fc receptor III; low affinity immunoglobulin gamma Fc region receptor II; lymphocyte antigen 17; RP11-5K23.1; unnamed protein product

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Gene Aliases: CD16; CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1

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UniProt ID: (Mouse) P08101

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Entrez Gene ID: (Mouse) 14130, (Mouse) 14131

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Function(s)
beta-amyloid binding protein binding IgG receptor activity low-affinity IgG receptor activity IgG binding immunoglobulin binding macromolecular complex binding immunoglobulin receptor binding
Process(es)
antibody-dependent cellular cytotoxicity negative regulation of type I hypersensitivity immune complex clearance negative regulation of immunoglobulin production negative regulation of inflammatory response to antigenic stimulus negative regulation of acute inflammatory response to antigenic stimulus negative regulation of humoral immune response mediated by circulating immunoglobulin receptor-mediated endocytosis phagocytosis, engulfment defense response cell surface receptor signaling pathway response to bacterium immunoglobulin mediated immune response dendrite development antigen processing and presentation of exogenous peptide antigen via MHC class II cerebellum development negative regulation of B cell proliferation endocytic recycling negative regulation of interleukin-10 production positive regulation of tumor necrosis factor production negative regulation of neutrophil apoptotic process Fc-gamma receptor signaling pathway positive regulation of JNK cascade regulation of long-term neuronal synaptic plasticity negative regulation of phagocytosis positive regulation of phagocytosis regulation of immune response negative regulation of immune response cellular response to molecule of bacterial origin Fc receptor-mediated immune complex endocytosis regulation of dendritic spine maintenance regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway cellular response to beta-amyloid positive regulation of response to endoplasmic reticulum stress positive regulation of type IIa hypersensitivity positive regulation of type III hypersensitivity positive regulation of type I hypersensitivity serotonin secretion phagocytosis, recognition neutrophil chemotaxis antigen processing and presentation of exogenous peptide antigen via MHC class I mast cell activation
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